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G1522 Sigma-Aldrich

Gentamicin solution

Hybri-Max, 50 mg/mL gentamicin, sterile-filtered, BioReagent, suitable for hybridoma

Synonym: Garamycin, Gentiomycin C

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Properties

Related Categories A - K, Aminoglycosides, Antibacterial, Antibiotic Solutions, Antibiotics,
grade   Hybri-Max
InChI Key   RDEIXVOBVLKYNT-HDZPSJEVSA-N
sterility   sterile-filtered
product line   BioReagent
form   liquid
quality   Prepared in tissue culture grade water.
concentration   50 mg/mL gentamicin
application(s)   cell culture | hybridoma: suitable
impurities   endotoxin, tested
suitability   suitable for hybridoma
Mode of action   protein synthesis | interferes
antibiotic activity spectrum   Gram-negative bacteria
  Gram-positive bacteria
  mycoplasma
storage temp.   2-8°C

Description

Components

Gentamicin is an aminoglycoside complex produced by fermentation of Micromonospora purpurea or M. echinospora. It is used as the sulfate salt. There are three components, each consisting of five basic nitrogens and requiresing five equivalents of sulfuric acid per mole of gentamicin base.

Application

Gentamicin sulfate is a broad-spectrum antibiotic used as a selection agent (gentamicin-resistance gene) in molecular biology and cell culture applications. This product is formulated to contain 50 mg/mL Gentamicin in deionized water and is recommended for use in eukaryotic cell culture at a volume of 1 milliliter per liter.

Biochem/physiol Actions

Mode of Action: Gentamicin inhibits protein synthesis by binding to the 30S subunit of the ribosome.

Antimicrobial spectrum: Includes Gram-negative and Gram-positive bacteria, including strains resistant to tetracycline, chloramphenicol, kanamycin and colistin, particularly strains of Pseudomonas, Proteus, Staphylococcus and Streptococcus.

Caution

The product is stable at 37° for 5 days and is stored at 2-8°C.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Price and Availability


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Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
nwg

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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G1397 Gentamicin solution, 50 mg/mL in deionized water, liquid, sterile-filtered, BioReagent, suitable for cell culture

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