Indo 1-AM ~95% (HPLC) | Sigma-Aldrich

Properties

Related Categories	Bioactive Small Molecules, Biochemicals and Reagents, Calcium Probes, Calcium Signaling Research Tools, Cell Biology, Cell Signaling and Neuroscience, Chelators and Ion Probes, Chelators and Ion Probes for Flow Cytometry, Chelators and Ion Probes for Microscopy, Chelators and Ion Probes for Spectroscopy, Fluorescent, Fluorescent Indicators and Probes, Fluorescent Probes, Labels, Particles and Stains, I, Intracellular Calcium Signaling, Luminescent Compounds/Detection, Wavelength Index More...
assay	~95% (HPLC)
fluorescence	λ_ex 330 nm; λ_em 450 nm (with Calcium)
	λ_ex 356 nm; λ_em 478 nm in methanol
storage temp.	−20°C

Description

Application

Cell permeable fluorescent probe for Ca²⁺; lower affinity than FURA 2; gives a large shift in fluorescent emission from 482 nm to 398 nm upon Ca²⁺ binding.

Price and Availability

Related Papers

The effects of pH and temperature on fluorescent calcium indicators as determined with Chelex-100 and EDTA buffer systems. Lattanzio Biochem. Biophys. Res. Commun. 171, 102, (1990)

Documents

Certificate of Analysis

Certificate of Origin

Safety Information

Personal Protective Equipment	Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
WGK Germany	3

Technical information & documentation associated with this product is available in the Safety & Documentation tab.

Articles

Sensor Dyes

In 1998 R.F. Furchgott, L.J. Ignarro, and F. Murad received the Nobel Prize in Physiology and Medicine for their discoveries concerning nitric oxide as a signaling molecule in the cardiovascular syst...
Monika Baeumle
BioFiles 2011, 6.3, 23.
Keywords: Biochemistry, Biological processes, Cancer, Cardiovascular, Central Nervous System, Diseases, Extinction coefficient, Indicators, Inflammation, Microscopy, Neurotransmission, Sol-gel