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I6758 Sigma-Aldrich

IPTG

≥99% (TLC), ≤0.1% Dioxane

Synonym: IPTG, Isopropyl β-D-1-thiogalactopyranoside, Isopropyl β-D-thiogalactoside

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Description

Application

IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.

Other Notes

Non-metabolizable galactose analog.

Packaging

1, 5, 10 g in glass bottle

Reconstitution

A stock solution (0.1 M) is prepared by dissolving IPTG in water with subsequent sterile filtration of the solution. The final concentration of IPTG in indicator plates should be 0.2 mM.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Protocols

Microbial Growth Protocols

From our library of Protocols, Sigma-Aldrich presents Microbial Growth Protocols
Keywords: Antibiotics

Transformation Protocols

From our library of Protocols, Sigma-Aldrich presents Transformation Protocols
Keywords: Antibiotics, Detergents, Electrophoresis, Gel electrophoresis, Mass spectrometry, Peptide synthesis, transformation

Peer-Reviewed Papers
15

References

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