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I8377 Sigma-Aldrich

Iodonitrotetrazolium chloride

Used in colorimetric assays.

Synonym: 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride, p-Iodonitrotetrazolium Violet, INT



Related Categories Cell Biology, Core Bioreagents, G-H-I, Hematology and Histology, Research Essentials,
assay   ≥98% (TLC)
form   powder
mp   240 °C (dec.)(lit.)
solubility   H2O: soluble4 mg/mL
storage temp.   2-8°C


Frequently Asked Questions

Frequently Asked Questions are available for this Product.


Electron acceptor for the colorimetric assay of various dehydrogenases

Iodonitrotetrazolium chloride has been used for the staining of cells. It has been used as a coupling agent in the enzymatic assay of D-arabinitol.


1, 5, 25 g in glass bottle

250 mg in glass bottle

Price and Availability

Tissue Diagnostics

All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
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A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
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What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
Product I8377, Iodonitrotetrazolium chloride, is stated to have solubility in water, ethanol and ethylene glycol monomethylether.  What other solutions can be used?
Conn's Biological Stains, 10th Edition (page 163) states P-Iodonitrotetrazolium Violet is also soluble in dimethylsulfoxide and dimethylsulfoxide-water mixtures.
Can Product I8377, Iodonitrotetrazolium chloride, be used for the staining of cells?
Yes, although due to the large crystal size of the formazan and lack of substantivity, histological application of I8377 has been limited.  In microbiology, the product was used by Lussenhop and Fogel (1993) for in situ microscopic observation of soil biota.. Gribbon and Barer (1995) used for the demonstration of viable but nonculturable bacteria.  Heidelberg (1997) used as a bacterial viability stain.
Is Product I8377, Iodonitrotetrazolium chloride, lipophilic?
Yes, I8377  is a lipophilic cation of moderate size.  The resulting formazan is more strongly liophilic and fat soluble.
Is there a procedure for reducing Product I8377, Iodonitrotetrazolium chloride, by superoxide radicals?
Yes.  See Biochem Biophys Res Commun. 1988 Feb 15;150(3):1294-301.
Is there a reference for using Product I8377, Iodonitrotetrazolium chloride, to treat brain membrane preparations?
Yes.  See Neuroscience Letters Volume 35, Issue 2, 21 February 1983, Pages 215-220
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Protocols & Articles
Peer-Reviewed Papers


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58030 Iodonitrotetrazolium chloride, BioReagent, ≥97.0% (calc. on dry substance, NT)

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