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M0178 Sigma-Aldrich

Middlebrook 7H9 Broth Base

for microbiology

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Properties

Related Categories A - M, All Liquid Media/Broths, Clinical Media, Media, Media by Application,
grade   for microbiology
shelf life   limited shelf life, expiry date on the label
final pH   6.6±0.2 (25 °C)
suitability   Mycobacteria

Description

Components

Ingredients (g/L)
Ammonium sulfate, 0.50
Disodium phosphate, 2.50
Monopotassium phosphate, 1.00
Sodium citrate, 0.10
Magnesium sulfate, 0.05
Calcium chloride, 0.0005
Zinc sulfate, 0.001
Copper sulfate, 0.001
Ferric ammonium citrate, 0.04
L-Glutamic acid, 0.50
Pyridoxine, 0.001
Biotin, 0.0005

Application

Recommended for cultivation and sensitivity testing of Mycobacterium tuberculosis.

Other Notes

Same product — same quality — now Sigma-Aldrich brand

Preparation Note

Supplements:
4 ml/l glycerol (Cat. No. 49767)
2 vial/l Middlebrook ADC Growth Supplement (Cat. No.M0553) or
1 g/l of Tween® 80 (Cat. No. P8074)
2 vial/l Middlebrook ADC Growth Supplement (Cat. No. M0553)

Legal Information

TWEEN is a registered trademark of Croda International PLC

Price and Availability


Sterifil Aseptic System and Holder
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the environment, microbes have adapted to the habitats most suitable for thei...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Peer-Reviewed Papers
15

References

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M0553 Middlebrook ADC Growth Supplement, for microbiology

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