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M0303 Sigma-Aldrich

Middlebrook 7H10 Agar Base

for microbiology

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Properties

Related Categories A - M, All Solid Media/Agars, Clinical Media, Media, Media by Application,
grade   for microbiology
shelf life   limited shelf life, expiry date on the label
final pH   6.6±0.2 (25 °C)
suitability   Mycobacteria

Description

Components

Ingredients (g/L)
Ammonium sulfate, 0.50
L-Glutamic acid, 0.50
Monopotassium phosphate, 1.50
Disodium phosphate, 1.50
Sodium citrate, 0.40
Ferric ammonium citrate, 0.04
Magnesium sulfate, 0.025
Calcium chloride, 0.0005
Zinc sulfate, 0.001
Copper sulfate, 0.001
Pyridoxine hydrochloride, 0.001
Biotin, 0.0005
Malachite green, 0.00025
Agar, 15.00

Application

Recommended for isolation, cultivation and sensitivity testing of Mycobacterium tuberculosis.

Other Notes

Same product — same quality — now Sigma-Aldrich brand

Preparation Note

Supplements:
5 ml/l Glycerol (Cat. No. 49767)
2 vial/l Middlebrook OADC Growth Supplement (Cat. No. M0678)

Price and Availability


Sterifil Aseptic System and Holder
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the environment, microbes have adapted to the habitats most suitable for thei...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Peer-Reviewed Papers
15

References

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M0678 Middlebrook OADC Growth Supplement, for microbiology

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