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M9140 Sigma-Aldrich

Methylene blue

certified by the Biological Stain Commission

Synonym: 3,7-bis(Dimethylamino)phenazathionium chloride, Basic Blue 9, Tetramethylthionine chloride

  • CAS Number 122965-43-9

  • Empirical Formula (Hill Notation) C16H18ClN3S · xH2O

  • Molecular Weight 319.85 (anhydrous basis)

  •  Beilstein Registry Number 3599847

  •  Colour Index Number 52015

  •  EC Number 200-515-2

  •  MDL number MFCD00150006

  •  PubChem Substance ID 24897317

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Properties

Related Categories Cell Biology, Certified Stains & Dyes, Core Bioreagents, Hematology and Histology, Life Science Reagents for DNA/RNA Electrophoresis,
grade   certified by the Biological Stain Commission
form   powder
composition   Dye content, ≥82%
solubility   H2O: soluble4mg/4ml
Gene Information   human ... CYP1A2(1544), GSR(2936)

Description

Frequently Asked Questions

Live Chat and Frequently Asked Questions are available for this Product.

Packaging

25, 100 g in glass bottle

Suitability

Certified for use in Mallory′s phloxine-methylene blue stain; Levine & Black stain for bacteria in milk; Wright stain for blood smears.

Price and Availability

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
WGK Germany 
3
RTECS 
SP5740000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers

References

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Performance of different three-dimensional scaffolds for in vivo endochondral bone generation. Yang W, Both SK, van Osch GJ, et al. Eur. Cell. Mater. 27, 350-64, (2014)

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Interfering with the interaction between ErbB1, nucleolin and Ras as a potential treatment for glioblastoma. Goldshmit Y, Trangle SS, Kloog Y, et al. Oncotarget 5(18), 8602-13, (2014)

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Sensory-specific modulation of adult neurogenesis in sensory structures is associated with the type of stem cell present in the neurogenic niche of the zebrafish brain. Lindsey BW, Di Donato S, Kaslin J, et al. Eur. J. Neurosci. 40(11), 3591-607, (2014)

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Acetoin synthesis acquisition favors Escherichia coli growth at low pH. Vivijs B, Moons P, Aertsen A, et al. Appl. Environ. Microbiol. 80(19), 6054-61, (2014)

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A yeast-based assay identifies drugs that interfere with immune evasion of the Epstein-Barr virus. Voisset C, Daskalogianni C, Contesse MA, et al. Dis. Model Mech. 7(4), 435-44, (2014)

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Metabolic programming of mesenchymal stromal cells by oxygen tension directs chondrogenic cell fate. Leijten J, Georgi N, Moreira Teixeira L, et al. Proc. Natl. Acad. Sci. U. S. A. 111(38), 13954-9, (2014)

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Epoxy alcohol synthase of the rice blast fungus represents a novel subfamily of dioxygenase-cytochrome P450 fusion enzymes. Hoffmann I, Jernerén F, Oliw EH, et al. J. Lipid Res. 55(10), 2113-23, (2014)

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Chitosan/glucose 1-phosphate as new stable in situ forming depot system for controlled drug delivery. Supper S, Anton N, Boisclair J, et al. Eur. J. Pharm. Biopharm. 88(2), 361-73, (2014)

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TgaA, a VirB1-like component belonging to a putative type IV secretion system of Bifidobacterium bifidum MIMBb75. Guglielmetti S, Balzaretti S, Taverniti V, et al. Appl. Environ. Microbiol. 80(17), 5161-9, (2014)

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Overcoming endocrine resistance due to reduced PTEN levels in estrogen receptor-positive breast cancer by co-targeting mammalian target of rapamycin, protein kinase B, or mitogen-activated protein kinase kinase. Fu X, Creighton CJ, Biswal NC, et al. Breast Cancer Res. 16(5), 430, (2015)

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Gold-mercaptopropionic acid-polyethylenimine composite based DNA sensor for early detection of rheumatic heart disease. Singh S, Kaushal A, Khare S, et al. Analyst 139(14), 3600-6, (2014)

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Sulfurized limonite as material for fast decomposition of organic compounds by heterogeneous Fenton reaction. Toda K, Tanaka T, Tsuda Y, et al. J. Hazard. Mater. 278, 426-32, (2014)

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Enrichment of reticulocytes from whole blood using aqueous multiphase systems of polymers. Kumar AA, Lim C, Moreno Y, et al. Am. J. Hematol. 90(1), 31-6, (2015)

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Regionally-specified second trimester fetal neural stem cells reveals differential neurogenic programming. Fan Y, Marcy G, Lee ES, et al. PLoS ONE 9(9), e105985, (2014)

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Suppression of the DHX9 helicase induces premature senescence in human diploid fibroblasts in a p53-dependent manner. Lee T, Di Paola D, Malina A, et al. J. Biol. Chem. 289(33), 22798-814, (2014)

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Platelet-rich plasma and bone defect healing. Malhotra A, Pelletier M, Oliver R, et al. Tissue Eng. Part A 20(19-20), 2614-33, (2014)

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An open source image processing method to quantitatively assess tissue growth after non-invasive magnetic resonance imaging in human bone marrow stromal cell seeded 3D polymeric scaffolds. Leferink AM, Fratila RM, Koenrades MA, et al. PLoS ONE 9(12), e115000, (2014)

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Alternative splicing of TAF6: downstream transcriptome impacts and upstream RNA splice control elements. Kamtchueng C, Stébenne MÉ, Delannoy A, et al. PLoS ONE 9(7), e102399, (2014)

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Porcine synapsin 1: SYN1 gene analysis and functional characterization of the promoter. Hedegaard C, Kjaer-Sorensen K, Madsen LB, et al. FEBS Open Bio 3, 411-20, (2013)

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RFTS-deleted DNMT1 enhances tumorigenicity with focal hypermethylation and global hypomethylation. Wu BK, Mei SC, Brenner C, et al. Cell Cycle 13(20), 3222-31, (2014)

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Sampling reliability, spatial resolution, spatial precision, and extraction efficiency in droplet-based liquid microjunction surface sampling. Kertesz V, Van Berkel GJ, et al. Rapid Commun. Mass Spectrom. 28(13), 1553-60, (2014)

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RNA-seq profiling of a radiation resistant and radiation sensitive prostate cancer cell line highlights opposing regulation of DNA repair and targets for radiosensitization. Young A, Berry R, Holloway AF, et al. BMC Cancer 14, 808, (2014)

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C/EBP homologous protein inhibits tissue repair in response to gut injury and is inversely regulated with chronic inflammation. Waldschmitt N, Berger E, Rath E, et al. Mucosal Immunol. 7(6), 1452-66, (2014)

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Interactomics profiling of the negative regulatory function of carbon monoxide on RANKL-treated RAW 264.7 cells during osteoclastogenesis. Tseng FJ, Chia WT, Shyu JF, et al. BMC Syst. Biol. 8, 57, (2014)

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S100A4 and uric acid promote mesenchymal stromal cell induction of IL-10+/IDO+ lymphocytes. Eisenbacher JL, Schrezenmeier H, Jahrsdörfer B, et al. J. Immunol. 192(12), 6102-10, (2014)

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Modified activin receptor IIB ligand trap mitigates ineffective erythropoiesis and disease complications in murine ?-thalassemia. Suragani RN, Cawley SM, Li R, et al. Blood 123(25), 3864-72, (2014)

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Interrogation of gossypol therapy in glioblastoma implementing cell line and patient-derived tumour models. Jarzabek MA, Amberger-Murphy V, Callanan JJ, et al. Br. J. Cancer 111(12), 2275-86, (2014)

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Tumor necrosis factor-like weak inducer of apoptosis or Fn14 deficiency reduce elastase perfusion-induced aortic abdominal aneurysm in mice. Tarín C, Fernández-Laso V, Sastre C, et al. J. Am. Heart Assoc. 3(4), doi:10.1161/JAHA.113.000723, (2014)

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Chronic alcohol consumption leads to neurochemical changes in the nucleus accumbens that are not fully reversed by withdrawal. Pereira PA, Neves J, Vilela M, et al. Neurotoxicol. Teratol. 44, 53-61, (2014)

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Involvement of Nod2 in the innate immune response elicited by malarial pigment hemozoin. Corbett Y, Parapini S, D'Alessandro S, et al. Microbes Infect. 17(3), 184-94, (2015)

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miR-148a- and miR-216a-regulated oncolytic adenoviruses targeting pancreatic tumors attenuate tissue damage without perturbation of miRNA activity. Bofill-De Ros X, Gironella M, Fillat C, et al. Mol. Ther. 22(9), 1665-77, (2014)

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Reversal of myofibroblastic activation by polyunsaturated fatty acids in valvular interstitial cells from aortic valves. Role of RhoA/G-actin/MRTF signalling. Witt W, Büttner P, Jannasch A, et al. J. Mol. Cell. Cardiol. 74, 127-38, (2014)

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Third-stage nematode larvae of Contracaecum osculatum from Baltic cod (Gadus morhua) elicit eosinophilic granulomatous reactions when penetrating the stomach mucosa of pigs. Strøm SB, Haarder S, Korbut R, et al. Parasitol. Res. 114(3), 1217-20, (2015)

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Sex-specific effects of relaxin-3 on food intake and brain expression of corticotropin-releasing factor in rats. Lenglos C, Calvez J, Timofeeva E, et al. Endocrinology 156(2), 523-33, (2015)

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Thoracic duct embolization and disruption for treatment of chylous effusions: experience with 105 patients. Pamarthi V, Stecker MS, Schenker MP, et al. J. Vasc. Interv. Radiol. 25(9), 1398-404, (2014)

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Organization of pontine reticulospinal inputs to motoneurons controlling axial and limb muscles in the neonatal mouse. Sivertsen MS, Glover JC, Perreault MC, et al. J. Neurophysiol. 112(7), 1628-43, (2014)

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Elevated FGF21 leads to attenuated postnatal linear growth in preterm infants through GH resistance in chondrocytes. Guasti L, Silvennoinen S, Bulstrode NW, et al. J. Clin. Endocrinol. Metab. 99(11), E2198-206, (2014)

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Effect of high-dose irradiation on human bone-marrow-derived mesenchymal stromal cells. Fekete N, Erle A, Amann EM, et al. Tissue Eng. Part C Methods 21(2), 112-22, (2015)

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Detecting multiple cell-secreted cytokines from the same aptamer-functionalized electrode. Liu Y, Liu Y, Matharu Z, et al. Biosens. Bioelectron. 64, 43-50, (2014)

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Antinociceptive effect of ethanol extract of leaves of Lannea coromandelica. Imam MZ, Moniruzzaman M, et al. J. Ethnopharmacol. 154(1), 109-15, (2014)

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Tfap2a promotes specification and maturation of neurons in the inner ear through modulation of Bmp, Fgf and notch signaling. Kantarci H, Edlund RK, Groves AK, et al. PLoS Genet. 11(3), e1005037, (2015)

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Ion-pair chromatography coupled to inductively coupled plasma-mass spectrometry (IPC-ICP-MS) as a method for thiomolybdate speciation in natural waters. Lohmayer R, Reithmaier GM, Bura-Naki? E, et al. Anal. Chem. 87(6), 3388-95, (2015)

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Dietary cholesterol directly induces acute inflammasome-dependent intestinal inflammation. Progatzky F, Sangha NJ, Yoshida N, et al. Nat. Commun. 5, 5864, (2014)

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In vitro characterization of self-assembled anterior cruciate ligament cell spheroids for ligament tissue engineering. Hoyer M, Meier C, Breier A, et al. Histochem. Cell Biol. 143(3), 289-300, (2015)

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Endothelium-dependent and -independent relaxation of rat aorta induced by extract of Schizophyllum commune. Chen H, Li S, Wang P, et al. Phytomedicine 21(11), 1230-6, (2014)

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A high-affinity, high-stability photoacoustic agent for imaging gastrin-releasing peptide receptor in prostate cancer. Levi J, Sathirachinda A, Gambhir SS, et al. Clin. Cancer Res. 20(14), 3721-9, (2014)

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