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M9929 Sigma-Aldrich

Methotrexate

meets USP testing specifications

Synonym: 4-Amino-10-methylfolic acid hydrate, L-4-Amino-N10-methylpteroylglutamic acid hydrate, L-Amethopterin hydrate, Antifolan hydrate, MTX hydrate, Methotrexate hydrate, Methylaminopterin hydrate

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Properties

Related Categories Apoptosis Reagents, Apoptosis and Cell Cycle, Cell Biology, Cell Culture, Cell Signaling and Neuroscience,
InChI Key   FPJYMUQSRFJSEW-ZOWNYOTGSA-N
suitability   meets USP testing specifications
storage temp.   −20°C
Gene Information   human ... DHFRP1(573971), FPGS(2356), SLC19A1(6573), TYMS(7298)
mouse ... Dhfr(13361)
rat ... Dhfr(24312)

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Biochem/physiol Actions

Methotrexate is an allosteric inhibititor of dihydrofolate reductase (DHFR), the enzyme that catalyzes the conversion of dihydrofolate to tetrahydrofolate. Since tetrahydrolfolate is required for purine and pyrimidine synthesis, methotrexate treatment results in the inhibition of DNA and RNA synthesis.

Packaging

25, 100, 500 mg in poly bottle

Application

Potent inhibitor of dihydrofolate reductase and agent for antitumor studies. Use to inhibit dihydrofolate reductase in DHFR-based protein expression systems. Also effective in treatment of pyrimethamine-resistant Plasmodium vivax malaria parasites.

Potent inhibitor of dihydrofolate reductase and agent for antitumor studies. Use to inhibit dihydrofolate reductase in DHFR-based protein expression systems. Also shows immunosuppressive effects in, e.g., rheumatoid arthritis.

Price and Availability


Laboratory Gloves

All labs need water
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN 1544PSN1 6.1 / PGIII
WGK Germany 
3
RTECS 
MA1225000

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
What is the solubility of Methotrexate, Product M9929?
Methotrexate is practically insoluble in water.  It does dissolve in solutions of mineral acids (such as HCl) and in dilute solutions of alkali hydroxides. A solution can be prepared in 0.1 M sodium hydroxide at 20 mg/ml (may require some heating).
Can Product M9929, Methotrexate, be stored?
Alkaline stock solutions can decompose   It is best to prepare stock solutions in a minimal amount of of 1 M sodium hydroxide, then dilute with saline or medium. Solutions can be stored at -20°C for about one month. Solutions can be stored for about 1 week at 2-8°C.
How can I sterilize Product M9929, Methotrexate solutions?
Solutions can be sterile filtered.
What does the description of "meets USP testing specification" signify?
The product has been tested according to the specifications in the monograph of the United States Pharmacopoeia.  Our specification sheet at our website lists the testing done. http://www.sigmaaldrich.com/catalog/DataSheetPage.do?brandKey=SIAL&symbol=M9929
Has Product M9929, Methotrexate, been tested for cell culture applications?
Product M9929 has not been cell culture tested. Product M8407, Methotrexate hydrate, has been both mammalian and insect cell culture tested and is recommended to use for these applications.
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Protocols & Articles

Articles

HPLC Analysis of Polyglutamate Metabolites of Methotrexate on TSKgel® NH2-100 and ODS-100V

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Polyglutamate Metabolites of Methotrexate on TSKgel® NH2-100 and ODS-100V
Keywords: Chromatography, High performance liquid chromatography, Metabolites, Separation

Nucleotide Synthesis in Cancer Cells

Neoplastic cells are highly dependent on the de novo synthesis of nucleotides to maintain sufficient pools to support DNA replication and the production of RNA. The metabolic pathways supporting nucl...
Keywords: Biofiles, Cancer, Catalysis, Citric Acid Cycle, DNA replication, Gene expression, Glycolysis, Metabolic Pathways, Metabolism, Metabolites, Nucleotide Synthesis, PAGE, Pentose phosphate pathway, Pyrimidine synthesis, Support

UHPLC Analysis of Methotrexate and Related Compounds on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of Methotrexate and Related Compounds on Ascentis® Express C18
Keywords: Clinical, Pharmaceutical, Separation

Peer-Reviewed Papers
15

References

Related Products

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Description

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06693 Timestrip Plus -20 °C
M8407 Methotrexate hydrate, powder, BioReagent, suitable for cell culture, ≥98% (HPLC)

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