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M9929 Sigma-Aldrich

Methotrexate

meets USP testing specifications

Synonym: 4-Amino-10-methylfolic acid hydrate, L-4-Amino-N10-methylpteroylglutamic acid hydrate, L-Amethopterin hydrate, Antifolan hydrate, MTX hydrate, Methotrexate hydrate, Methylaminopterin hydrate

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Properties

Related Categories Apoptosis Reagents, Apoptosis and Cell Cycle, Cell Biology, Cell Culture, Cell Signaling and Neuroscience,
InChI Key   FPJYMUQSRFJSEW-ZOWNYOTGSA-N
suitability   meets USP testing specifications
storage temp.   −20°C
Gene Information   human ... DHFRP1(573971), FPGS(2356), SLC19A1(6573), TYMS(7298)
mouse ... Dhfr(13361)
rat ... Dhfr(24312)

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Biochem/physiol Actions

Methotrexate is an allosteric inhibititor of dihydrofolate reductase (DHFR), the enzyme that catalyzes the conversion of dihydrofolate to tetrahydrofolate. Since tetrahydrolfolate is required for purine and pyrimidine synthesis, methotrexate treatment results in the inhibition of DNA and RNA synthesis.

Packaging

25, 100, 500 mg in poly bottle

Application

Potent inhibitor of dihydrofolate reductase and agent for antitumor studies. Use to inhibit dihydrofolate reductase in DHFR-based protein expression systems. Also effective in treatment of pyrimethamine-resistant Plasmodium vivax malaria parasites.

Potent inhibitor of dihydrofolate reductase and agent for antitumor studies. Use to inhibit dihydrofolate reductase in DHFR-based protein expression systems. Also shows immunosuppressive effects in, e.g., rheumatoid arthritis.

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.




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Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN 1544PSN1 6.1 / PGIII
WGK Germany 
3
RTECS 
MA1225000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Nucleotide Synthesis in Cancer Cells

Neoplastic cells are highly dependent on the de novo synthesis of nucleotides to maintain sufficient pools to support DNA replication and the production of RNA. The metabolic pathways supporting nucl...
Keywords: Biofiles, Cancer, Catalysis, Citric Acid Cycle, DNA replication, Gene expression, Glycolysis, Metabolic Pathways, Metabolism, Metabolites, Nucleotide Synthesis, PAGE, Pentose phosphate pathway, Pyrimidine synthesis, Support

Peer-Reviewed Papers
15

References

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Related Products

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Product #

Description

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06693 Timestrip Plus -20 °C
M8407 Methotrexate hydrate, powder, BioReagent, suitable for cell culture, ≥98% (HPLC)

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