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N6639 Sigma-Aldrich

Nitrotetrazolium Blue chloride

powder, electrophoresis grade

Synonym: 2,2′-bis(4-Nitrophenyl)-5,5′-diphenyl-3,3′-(3,3′-dimethoxy-4,4′-diphenylene)ditetrazolium chloride, 3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], p-Nitro-Blue tetrazolium chloride, p-Nitrotetrazolium blue, NBT, Nitro BT

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Properties

Related Categories Cell Biology, Colorimetric, Hematology and Histology, Molecular Biology, N,
grade   electrophoresis grade
InChI Key   FSVCQIDHPKZJSO-UHFFFAOYSA-L
assay   >97%
form   powder
solubility   H2O: 10 mg/mL (A stock solution at 10mg/ml in water is stable for 1-2 weeks in the dark at 0-4°C.)
  2-methoxyethanol: 20 mg/mL
  ethanol: 5 mg/mL
storage temp.   2-8°C

Description

Packaging

1 g in glass bottle

50, 250 mg in glass insert

Application

Nitrotetrazolium blue (NBT), in combination with BCIP, is used in detection of nonradioactively labeled probes after the hybridization step in Southern, Northern, and Western blots. Part of the colorimetric detection assay, NBT and BCIP dyes detect alkaline phosphatase activity, producing an insoluble purple dye (diformazan) that becomes visible at sites where the labeled probe has hybridized to its target.

General description

Nitrotetrazolium Blue chloride (NBT) is a dye that is used for detection of alkaline phosphatase in combination with 5-bromo-4-chloro-3-indoxyl phosphate (BCIP).

Price and Availability


Tissue Diagnostics

All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
XF8045000
Protocols & Articles

Articles

Intro to Detection Methods Used in Molecular Biology

Different detection methods are used to visualize the nucleic acids and specific proteins transferred to membrane support. Southern and northern blotting involves the use of radioactive probes and th...
Keywords: Catalytic combustion detector, Detection methods, Northern blot, Nucleic acid hybridization, Purification, Size-exclusion chromatography, Western blot

Protocols

Nitrotetrazolium blue chloride for molecular biology protocol

CAS RN 298-83-9 EC 206-067-4 Synonyms: p-Nitro-Blue tetrazolium chloride, p-nitrotetrazolium blue, NBT, Nitro BT
Keywords: Extinction coefficient, Indicators, Melting, Western blot

Peer-Reviewed Papers
15

References

Related Products

related product

Product #

Description

Add to Cart

11383221001 BCIP, 4-toluidine salt
BCIPP-RO BCIP, 5-bromo-4-chloro-3-indolyl-phosphate, 4-toluidine salt
I9516 2-Propanol, BioReagent, for molecular biology, ≥99.5%

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