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P0914 Sigma-Aldrich

Protein standard

Micro Standard, liquid

Synonym: Albumin from bovine serum, protein standard

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Protein standard for all protein assays. Sealed ampules provide assurance that the concentration will be correct with every assay.

Concentrated solution for all protein assays.

Other Notes

1 mg bovine serum albumin/ml in 0.15 M NaCl

Packaging

5, 10 amp in ampule

Sealed ampules

Suitability

This product is commonly used for protein determination by the Bicinchoninic Acid (BCA) method.

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
Do you have any information on the traceability to a recognized protein standard, such as NIST? Or is the concentration derived from accepted values of natural physical constants?
The concentration of each lot of the protein standard, Product P0914, is determined by UV measurements. Specifically, given a known extinction coefficient (E) and a measured absorbance (A), and assuming a standard pathlength of 1 cm, the concentration (c) can be determined by Beer's Law (c = A/E). The assay result is derived from an accepted value of a natural physical constant. Specifically, the E1% of 6.67 at approximately 279 nm is a well-accepted value, originally published in J. Am. Chem. Soc., 78, 3656-3660 (1956).
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Protocols & Articles

Articles

HPLC Analysis of Four Proteins on Zenix® SEC-300, Comparison of Column Internal Diameter

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Four Proteins on Zenix® SEC-300, Comparison of Column Internal Diameter
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Protein Standards by Size Exclusion on Zenix® as Affected by Pore Size: 100Å vs. 300Å

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Protein Standards by Size Exclusion on Zenix® as Affected by Pore Size: 100Å vs. 300Å
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Protein Standards on Zenix® SEC-300 at Low Flow Rate

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Protein Standards on Zenix® SEC-300 at Low Flow Rate
Keywords: Chromatography, Diffusion, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Protein Standards on Zenix® SEC-300 by Size Exclusion As Affected by Arginine

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Protein Standards on Zenix® SEC-300 by Size Exclusion As Affected by Arginine
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Protein Standards on Zenix® SEC-300 by Size Exclusion As Affected by Potassium Chloride

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Protein Standards on Zenix® SEC-300 by Size Exclusion As Affected by Potassium Chloride
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Protein Standards on Zenix® SEC-300 by Size Exclusion As Affected by Sodium Perchlorate

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Protein Standards on Zenix® SEC-300 by Size Exclusion As Affected by Sodium Perchlorate
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Protein Standards on Zenix® SEC-300 in the Presence of a Denaturant

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Protein Standards on Zenix® SEC-300 in the Presence of a Denaturant
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Protein Standards on Zenix® SEC-300 using Different Buffer Systems

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Protein Standards on Zenix® SEC-300 using Different Buffer Systems
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Proteins (2) on BIOshell™ A400 Protein C4

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins (2) on BIOshell™ A400 Protein C4
Keywords: Chromatography, High performance liquid chromatography, Reversed-phase chromatography

HPLC Analysis of Proteins by Size Exclusion on Zenix® Columns, Effect of Pore Size

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins by Size Exclusion on Zenix® Columns, Effect of Pore Size
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Proteins by Size Exclusion on Zenix® SEC-300 Columns, Effect of Flow Rate (15 cm column)

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins by Size Exclusion on Zenix® SEC-300 Columns, Effect of Flow Rate (15 cm column)
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Proteins by Size Exclusion on Zenix® SEC-300 Columns, Effect of Flow Rate (30 cm column)

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins by Size Exclusion on Zenix® SEC-300 Columns, Effect of Flow Rate (30 cm column)
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Proteins by Size Exclusion on Zenix® and SRT® SEC-300, Comparison of Particle Size and Column I.D.

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins by Size Exclusion on Zenix® and SRT® SEC-300, Comparison of Particle Size and Column I.D.
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Proteins on BIOshell™ A400 Protein C18, Hydrophobicity Comparision

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on BIOshell™ A400 Protein C18, Hydrophobicity Comparision
Keywords: High performance liquid chromatography

Protein Molecular Weight Calibration Curve on Zenix® Size Exclusion Columns, Effect of Pore Size

From our library of Articles, Sigma-Aldrich presents Protein Molecular Weight Calibration Curve on Zenix® Size Exclusion Columns, Effect of Pore Size
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

Protocols

Protein Determination Modified Lowry Method

1. OBJECTIVE To standardize a procedure for the determination of protein by modified Lowry at Sigma-Aldrich, St. Louis, MO.
Keywords: Biochemistry, Lowry assay, Precipitation, Protein assay

Peer-Reviewed Papers
15

References

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