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P0972 Sigma-Aldrich

Polymyxin B sulfate

meets USP testing specifications, powder

Synonym: Polymyxin B sulfate salt

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Properties

Related Categories Analytical/Chromatography, Antibacterial, Antibiotic powders, Antibiotics, Antibiotics A to Z,
form   powder
solubility   H2O: soluble50 mg/mL (Stock solutions should be filter sterilized and stored at 2-8 °C. Stable at 37 °C for 5 days.)
suitability   meets USP testing specifications
Mode of action   cell membrane | interferes
storage temp.   2-8°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Polymyxin B sulfate is a strongly cationic cyclic polypeptide antibiotic that is derived from fermentation of Bacilus polymyxa. It is a mixture of B1 and B2 sulfate. The product has been used clinically to treat infections of the urinary tract, meninges and blood stream caused by susceptible strains of Pseudomonas aeruginosa. It also has uses studying multidrug-resistant pathogens, as an immobilized agent for removal of endotoxins, and to induce pore formation in the membranes of cortex cells from excised sorghum roots.

Biochem/physiol Actions

Mode of Action: Polymyxin B Sulfate binds to the lipid A portion of bacterial lipopolysaccharides†, disrupting the cytoplasmic membrane by inducing pores large enough to permit nucleotide leakage in bacterial walls. This disrupts the permeability of the cytoplasmic membrane.

Antimicrobial spectrum: Has bactericidal action on most gram-negative bacilli††, including E. Coli and on most fungi and gram-positive bacteria.

Caution

As supplied, the product should be stored at 2-8°C in the dark. No change was observed in retained samples after three years′ of storage in these conditions. Stock solutions should be sterile filtered and stored at 2-8°C. They are stable at 37°C for 5 days.

Packaging

This product is packaged in a bottomless glass bottle, with its contents inside an inserted fused cone.

Preparation Note

Polymyxin B sulfate is soluble in water at 50 mg/mL, producing a very slightly hazy, colorless to yellow solution. It has only minimal solubility in any organic solvent. For example, it has a solubility of 0.115 mg/mL in ethanol.

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Laboratory Gloves

All labs need water
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
TR1150000

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
What is MU and how does this relate to milligrams?
MU is million units. You can convert this to mg by dividing package size by the unit activity listed on the Certificate of Analysis for your specific lot.
What is the solution stability of Product P0972, Polymyxin B sulfate?
For solution stability information, please refer to the product information sheet for a related Polymyxin B sulfate product (Product No. P1004). You may open this document by clicking on the following link: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma-Aldrich/Product_Information_Sheet/p1004pis.Par.0001.File.tmp/p1004pis.pdf
I see that you list several Polymyxin B Sulfate products (P0972, P1004, P4932, 81334 and P4119). What are the differences?
The primary difference between the products is the testing. Product P0972 is USP tested, product P4932 is tested against 3 mammalian cell lines to make sure it is not toxic to the cells, product P4119 is cell culture and endotoxin tested, and products P1004 and 81334 are the research grades with P1004 in USP units and 81334 in international units.  
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles
Peer-Reviewed Papers
15

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