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P1379 Sigma-Aldrich

TWEEN® 20

viscous liquid

Synonym: Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate

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Properties

Related Categories Antigen-Vaccine Preparation, Biochemicals and Reagents, Buffers, Cell Biology, Cell Culture,
Quality Level   PREMIUM
description   non-ionic
form   viscous liquid
mol wt   mol wt ~1228
composition   lauric acid, ≥40% (balance primarily myristic, palmitic, and stearic acids)
impurities   ≤3.0% water
refractive index   n20/D 1.468(lit.)
CMC   0.06 mM(20-25°C)
acid number   ≤2.2 mg/g
hydroxyl value   96‑108 mg/g
transition temp   cloud point 76 °C
density   1.095 g/mL at 25 °C(lit.)
HLB   16.7
suitability   complies for IR spectroscopy

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Non-ionic detergent

TWEEN 20 has been used as a component of:
• washing buffer and blocking buffer in Western blotting
• blocking buffer in immunohistochemistry
• washing buffer in ELISA (enzyme linked immunosorbent assay)
• reaction mixture in PCR (polymerase chain reaction)

TWEEN 20 is a nonionic detergent widely used in biochemical applications. It has been used as an emulsifying agent for the preparation of stable oil-in-water emulsions. TWEEN 20 has been used in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins).
TWEEN 20 has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.005 to 0.5%.

Caution

TWEEN 20 may not be stable to autoclaving, particularly with metal cations in buffer solutions. Autoclaving is not recommended without testing for changes in properties. TWEEN 20 is heat sensitive and will darken when exposed to elevated temperatures. Polysorbates have been reported to be incompatible with alkalis, heavy metal salts, phenols, and tannic acid. They may also reduce the activity of many preservatives. Aqueous solutions of polysorbates undergo autoxidation during storage, with changes being catalyzed by light, increased temperature, and copper sulfate.

Packaging

1, 4 L in poly bottle

25, 100, 250, 500, 6×500 mL in poly bottle

Preparation Note

TWEEN 20 is miscible in water (100mg/mL), yielding a clear, yellow solution. It is also miscible with alcohol, dioxane, and ethyl acetate. TWEEN 20 is insoluble in liquid paraffin and fixed oils.

General description

TWEEN 20 is a nonionic detergent widely used in biochemical applications. It has been used as an emulsifying agent for the preparation of stable oil-in-water emulsions.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Price and Availability


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Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
TR7400000
Flash Point(F) 
>230 °F
Flash Point(C) 
>110 °C
Protocols & Articles
Peer-Reviewed Papers
15

References

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