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P3504 Sigma-Aldrich

Ponceau S

BioReagent, suitable for electrophoresis

Synonym: 3-Hydroxy-4-(2-sulfo-4-[4-sulfophenylazo]phenylazo)-2,7-naphthalenedisulfonic acid sodium salt

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Properties

Related Categories Buffers, Reagents, & Stains, Cell Biology, Core Bioreagents, General Reagents, Hematology and Histology,
grade   BioReagent
form   powder
solubility   H2O: soluble1 mg/mL
absorption   extinction/518-522 nm, deionized water ≥330000
suitability   suitable for electrophoresis

Description

Application

Ponceau S dye is used to make a stain for rapid reversible staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. The following are two common stain formulations;
0.1% Ponceau S (w/v) in 5% acetic acid
2% Ponceau S (w/v) in 30% TCA, 30% sulfosalicylic acid

Packaging

10, 50, 100 g in glass bottle

Price and Availability

TruPAGE™ Pre-cast Polyacrylamide Gels


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141194Ponceau S

Dye content 75 %

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
Hazard Codes (Europe) 
Xi
Risk Statements (Europe) 
Safety Statements (Europe) 
26-36
WGK Germany 
3
RTECS 
QJ6600000

Protocols & Articles

Peer-Reviewed Papers

References

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Scanning ultrasound removes amyloid-β and restores memory in an Alzheimer's disease mouse model. Leinenga G and Götz J Sci. Transl. Med. 7(278), 278ra33, (2015)

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Proinflammatory cytokines activate the intrinsic apoptotic pathway in β-cells. Lars G. Grunnet et al Diabetes 58, 1807-15, (2009)

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The IL-10R1 S138G loss-of-function allele and ulcerative colitis. Grundtner P, Gruber S, Murray SS, et al. Genes Immun. 10(1), 84-92, (2009)

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RNF41 (Nrdp1) controls type 1 cytokine receptor degradation and ectodomain shedding. Wauman, J., et al. J. Cell Sci. 124, 921-32, (2011)

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Sodium dodecyl sulphate-polyacrylamide denaturing gel electrophoresis and Western blotting techniques. Blancher C and Cormick RM Methods Mol. Biol. 878, 89-110, (2012)

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Interactions of hemoglobin in live red blood cells measured by the electrophoresis release test. Su Y, Gao L, and Qin W Methods Mol. Biol. 869, 393-402, (2012)

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Cationic electrophoresis. Buxbaum E Methods Mol. Biol. 869, 55-63, (2012)

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Algal decolorization and degradation of monoazo and diazo dyes. Omar HH Pak. J. Biol. Sci. 11(10), 1310-6, (2008)

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Detection of proteins on blot membranes. Harper S and Speicher DW Curr. Protoc. Protein Sci. Chapter 10, Unit 10.8, (2001)

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Immunoblotting and immunodetection. Gallagher S, Winston SE, Fuller SA, et al. Curr. Protoc. Neurosci. Chapter 5, Unit 5.19, (2004)

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Immunoblotting and immunodetection. Gallagher S, Winston SE, Fuller SA, et al. Curr. Protoc. Mol. Biol. Chapter 10, Unit 10.8, (2004)

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Immunoblotting and immunodetection. Gallagher SR, Winston SE, Fuller SA, et al. Curr. Protoc. Cell Biol. Chapter 6, Unit 6.2, (2001)

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Comparison of five methods for determination of total plasma protein concentration. Okutucu B, Dinçer A, Habib O, et al. J. Biochem. Biophys. Methods 70(5), 709-11, (2007)

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Fiducial markers for combined 3-dimensional mass spectrometric and optical tissue imaging. Chughtai K, Jiang L, Greenwood TR, et al. Anal. Chem. 84(4), 1817-23, (2012)

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O-GLcNAc post-translational modifications regulate the entry of neurons into an axon branching program. Francisco H Dev. Neurobiol. 69(2-3), 162-73, (2009)

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Western blotting as a method for studying cell-biomaterial interactions: the role of protein collection. van Kooten TG, Klein CL, and Kirkpatrick CJ J. Biomed. Mater. Res. 54(3), 385-9, (2001)

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HyperISGylation of Old World monkey ISG15 in human cells. Els Pattyn et al PLoS ONE 3, e2427, (2008)

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Retinoid metabolism and ALDH1A2 (RALDH2) expression are altered in the transgenic adenocarcinoma mouse prostate model. Touma SE Biochem. Pharmacol. 78(9), 1127-38, (2009)

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Problems with the estimation of urine protein by automated assays. Dube J, Girouard J, Leclerc P, et al. Clin. Biochem. 38(5), 479-85, (2005)

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The role of Akt/protein kinase B subtypes in retinal ischemic preconditioning. Dreixler JC, Hemmert JW, Shenoy SK, et al. Exp. Eye Res. 88(3), 512-21, (2009)

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Aquaporins play a role in desiccation and freeze tolerance in larvae of the goldenrod gall fly, Eurosta solidaginis Philip, B. et al. J. Exp. Biol. 211, 1114-1119, (2008)

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Uracil DNA N-glycosylase promotes assembly of human centromere protein A. Zeitlin SG, Chapados BR, Baker NM, et al. PLoS ONE 6(3), e17151, (2011)

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Potential protein toxicity of synthetic pigments: binding of poncean S to human serum albumin. Gao HW, Xu Q, Chen L, et al. Biophys. J. 94(3), 906-17, (2008)

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Responses of the insulin signaling pathways in the brown adipose tissue of rats following cold exposure. Wang X and Wahl R PLoS ONE 9(6), e99772, (2014)

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TRIM32 regulates skeletal muscle stem cell differentiation and is necessary for normal adult muscle regeneration. Sarah Nicklas et al PLoS ONE 7, e30445, (2012)

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The Swi2-Snf2-like protein Uls1 is involved in replication stress response. Cal-Bakowska M, Litwin I, Bocer T, et al. Nucleic Acids Res. 39(20), 8765-77, (2011)

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Biochemical analysis of cell-derived apoE3 particles active in stimulating neurite outgrowth. DeMattos RB, Rudel LL, and Williams DL J. Lipid Res. 42(6), 976-87, (2001)

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Robust methods for purification of histones from cultured mammalian cells with the preservation of their native modifications. Rodriguez-Collazo, P., et al. Nucleic Acids Res. 37(11), e81, (2009)

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Detection of phosphoproteins on electroblot membranes using a small-molecule organic fluorophore. Goodman T, Schulenberg B, Steinberg TH, et al. Electrophoresis 25(15), 2533-8, (2004)

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Equilibrium, kinetic and thermodynamic studies on the biosorption of reactive acid dye on Enteromorpha flexuosa and Gracilaria corticata. Sivasamy A, Nethaji S, and Nisha LL Environ. Sci. Pollut. Res. Int. 19(5), 1687-95, (2012)

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An Afg2/Spaf-related Cdc48-like AAA ATPase regulates the stability and activity of the C. elegans Aurora B kinase AIR-2. Heallen TR Dev. Cell 15(4), 603-16, (2008)

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Epithelial membrane protein-2 promotes endometrial tumor formation through activation of FAK and Src. Maoyong Fu et al PLoS ONE 6, e19945, (2011)

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Native red electrophoresis--a new method suitable for separation of native proteins. Dráb T, Kračmerová J, Tichá I, et al. Electrophoresis 32(24), 3597-9, (2011)

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Gene expression profiling of a mouse model of pancreatic islet dysmorphogenesis. Wilding Crawford, L PLoS ONE 3(2), e1611, (2008)

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Trifluoroacetylated proteins in liver and plasma of guinea pigs treated with HCFC-123 and halothane. Bortolato S, Zanovello A, Rugge M, et al. Toxicol. Lett. 144(1), 35-47, (2003)

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Tumor-suppressor activity of RRIG1 in breast cancer. Zhang G BMC Cancer 11, 32, (2011)

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Native polyacrylamide electrophoresis in the presence of Ponceau Red to study oligomeric states of protein complexes. Dráb T, Kračmerová J, Tichá I, et al. J. Sep. Sci. 34(14), 1692-5, (2011)

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Determination of malic Acid using a malate dehydrogenase reactor after purification and immobilization in non-denaturing conditions and staining with ponceau S. Shimazaki Y and Sakikawa T Protein Pept. Lett. 17(8), 1048-52, (2010)

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Upregulation of heme oxygenase-1 by degranulation in rat basophilic leukemia cells. Yasui Y, Sasao E, Sakata M, et al. Biol. Pharm. Bull. 30(3), 443-6, (2007)

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The 315-316 deletion determines the BXP-21 antibody epitope but has no effect on the function of wild type ABCG2 or the Q141K variant. Polgar O Mol. Cell Biochem. 322(1-2), 63-71, (2009)

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Quantifying protein in cerebrospinal fluid and urine: success achieved. Pesce MA Clin. Chem. 54(4), 770, (2008)

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High-efficiency staining of proteins on different blot membranes. Yonan CR, Duong PT, and Chang FN Anal. Biochem. 338(1), 159-61, (2005)

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FT-IR 1 (2), 989:B / FT-IR 2 (3), 4112:D / IR-Spectra (3), 1463:E / RegBook 1 (2), 2763:H / Sigma FT-IR 1 (2), 200:A / Stains and Dyes Ref, 583 / Structure Index 1, 438:C:6

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