P3504 Sigma-Aldrich

Ponceau S

BioReagent, suitable for electrophoresis

Synonym: 3-Hydroxy-4-(2-sulfo-4-[4-sulfophenylazo]phenylazo)-2,7-naphthalenedisulfonic acid sodium salt


Related Categories Buffers, Reagents, & Stains, Cell Biology, Core Bioreagents, General Reagents, Hematology and Histology,
grade   BioReagent
form   powder
solubility   H2O: soluble1 mg/mL
absorption   extinction/518-522 nm, deionized water ≥330000
suitability   suitable for electrophoresis



Ponceau S dye is used to make a stain for rapid reversible staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. The following are two common stain formulations;
0.1% Ponceau S (w/v) in 5% acetic acid
2% Ponceau S (w/v) in 30% TCA, 30% sulfosalicylic acid


10, 50, 100 g in glass bottle

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