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P3504 Sigma-Aldrich

Ponceau S

BioReagent, suitable for electrophoresis

Synonym: 3-Hydroxy-4-(2-sulfo-4-[4-sulfophenylazo]phenylazo)-2,7-naphthalenedisulfonic acid sodium salt

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Properties

Related Categories Buffers, Reagents, & Stains, Cell Biology, Core Bioreagents, General Reagents, Hematology and Histology,
grade   BioReagent
InChI Key   KQHKSGRIBYJYFX-WNDMICSESA-J
form   powder
solubility   H2O: 1 mg/mL
absorption   extinction/518-522 nm, deionized water ≥330000
suitability   suitable for electrophoresis

Description

Packaging

10, 50, 100 g in glass bottle

Application

Ponceau S has been used for membrane staining (Western blotting.

General description

Ponceau S is the sodium salt of a diazo dye. It is used to make a stain for rapid reversible staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. The stain is reversed with water washes and aids in subsequent immunological detection.
The following are two common stain formulations:
0.1% Ponceau S (w/v) in 5% acetic acid
2% Ponceau S (w/v) in 30% TCA, 30% sulfosalicylic acid

Price and Availability


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Tissue Diagnostics

Biomedical Applications
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
QJ6600000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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