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P5379 Sigma-Aldrich

Potassium phosphate monobasic

ReagentPlus®

Synonym: Monopotassium phosphate, Potassium dihydrogen phosphate, prim.-Potassium phosphate

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Properties

Related Categories Biochemicals and Reagents, Biological Buffers, Buffers A to Z More...
grade   ReagentPlus®
InChI Key   GNSKLFRGEWLPPA-UHFFFAOYSA-M
assay   ≥99.0%
pKa (25 °C)   (1) 2.15, (2) 6.82, (3) 12.38 (phosphoric acid)
mp   252.6 °C(lit.)
density   2.338 g/mL at 25 °C(lit.)

Description

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1 kg in poly bottle

10 kg in poly drum

100, 500 g in poly bottle

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Laboratory Gloves

Redi-Dri

Interactive Buffer Table
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
TC6615500

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Protocols

Enzymatic Assay of 3α-Hydroxysteroid Dehydrogenase with Androsterone (EC 1.1.1.50)

To standardize a procedure for the enzymatic determination of 3α-Hydroxysteroid dehydrogenase.
Keywords: Chromatography, Enzymology, Extinction coefficient

Enzymatic Assay of Alcohol Oxidase (EC 1.1.3.13)

The continuous spectrophotometric rate determination (A405, Light path = 1 cm) is based on the following reactions:
Keywords: Extinction coefficient

Enzymatic Assay of Cathepsin B

This procedure applies to all products that have a specification for Cathepsin B activity, such as Sigma-Aldrich Product Numbers C0150 and C8571, determined by the liberation of 7-amino-4-methylcouma...
Keywords: Enzyme activity, Enzymology

Enzymatic Assay of Cholesterol Oxidase

To standardize a procedure for the determination of the enzymatic assay of Cholesterol Oxidase.
Keywords: Enzyme activity, Extinction coefficient, Isomerizations

Enzymatic Assay of D-Alanine Racemase (EC 5.1.1.1)

To standardize a procedure for the enzymatic assay of D-Alanine Racemase, Sigma Product Number A8936, at Sigma-Aldrich St. Louis.
Keywords: Extinction coefficient

Enzymatic Assay of Ficin (EC 3.4.22.3)

The spectrophotometric stop rate detemination (A280, Light path = 1 cm) is based on the following reaction:

Enzymatic Assay of Fumarase

To standardize a procedure for the enzymatic assay of Fumarase in Analytical Services at Sigma-Aldrich - St. Louis.
Keywords: Extinction coefficient

Enzymatic Assay of Laccase (EC 1.10.3.2)

To standardize a procedure for the enzymatic assay of laccase at Sigma-Aldrich, St. Louis, Mo.

Enzymatic Assay of Lipase Type XIII from Pseudomonas Species and Pseudomonas Cepacia using a coupled enzyme system of Glycerol Kinase and Glycerophosphate Oxidase (EC 3.1.1.3)

To standardize a procedure for the enzymatic assay of Lipase Type XIII from Pseudomonas Species, Sigma Product Number L9518 , and Pseudomonas Cepacia, Sigma Product Number L9156, using a coupled enzy...
Keywords: Centrifugation, Extinction coefficient, High performance liquid chromatography

Enzymatic Assay of Lysozyme (EC 3.2.1.17)

This procedure may be used for Lysozyme products. The enzymatic rate determination (A450, Light path = 1 cm) is based on the following reaction:

Enzymatic Assay of Lyticase

This procedure may be used for the determination of Lyticase activity using Baker’s yeast as the substrate. The turbidimetric rate determination [Absorbance at 800 nm (A800), Light path = 1 cm] is ba...
Keywords: Enzyme activity, Sonication

Enzymatic Assay of Malic Dehydrogenase (EC 1.1.1.37)

The continuous spectrophotometric rate determination (A340, Light path = 1 cm) is based on the following reaction:
Keywords: Extinction coefficient

Enzymatic Assay of Peroxidase (EC 1.11.1.7) 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a Substrate

Replaces SPABTS02. Procedure updated to conform to current QUMAS formatting guidelines. Refer to CR SOP-DEK-ENZ42.
Keywords: Extinction coefficient

Enzymatic Assay of Phytase (EC 3.1.3.8)

Derived from procedure SSPHYT02. Includes template updates to current SOP specifications and incorporation of notes into the procedure. Refer to SOP-DEK-ENZ18.
Keywords: Biochemistry

Enzymatic Assay of Plasmin with D-Val-Leu-Lys-p-Nitroanilide Dihydrochloride

This scope of this procedure applies to products that have a specification for the enzymatic activity of plasmin.
Keywords: Enzyme activity, Extinction coefficient

Enzymatic Assay of Proteinase K (EC 3.4.21.64)

This procedure may be used for determination of Proteinase K activity using hemoglobin as the substrate. It is not used to assay the activity of immobilized Proteinase K products, such as Catalog Num...
Keywords: Immobilization

Enzymatic Assay of β-Glucuronidase (EC 3.2.1.31) from E. coli

The scope of this procedure is all products from E. coli that have a specification for β-Glucuronidase activity.
Keywords: Enzymology

Related Content

BioUltra Biological Buffers

A buffer, as defined by Van Slyke [1], is "a substance which by its presence in solution increases the amount of acid or alkali that must be added to cause unit change in pH". Buffers are thus very i...
Keywords: Absorption, Adsorption, Biochemistry, Biological Buffers, Biological processes, Buffers, PAGE

Peer-Reviewed Papers
15

References

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