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P7170 Sigma-Aldrich

Ponceau S solution

BioReagent, suitable for electrophoresis, 0.1 % (w/v) in 5% acetic acid

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Description

Application

Ponceau S stain comes ready-to-use and is designed for rapid (5 min) staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. Ponceau S staining on nitrocellulose and PVDF is reversible by washing the stained membrane with 0.1 M NaOH for 1 min. The stained and destained membrane can then be immunologically detected (Western blotting). A product information sheet is supplied which includes the staining procedure for cellulose acetate, nitrocellulose, and PVDF.

Ponceau S solution is the suitable reagent for use in electrophoresis studies. It was used as a stain to visualize the protein loading onto a nitrocellulose membrane during western blotting analysis. It was used as stain for the proteins transferred to poly (vinylidene) fluoride (PVDF) membranes during Western blot analysis. It may be employed as stain for the purified fibrinolytic enzyme (CK) for the analysis of N-terminal amino acid sequence of the first 14 residues of CK by a protein sequencer. It may be used as a stain during the reversible staining of transferred proteins and for the qualitative visualization of proteins on nitrocellulose and PVDF membranes during immunoblotting (western blotting) studies.

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Safety Information

RIDADR 
NONH for all modes of transport
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Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Electrophoresis - Protein Staining Reagents

To meet the great diversity of protein analysis needs, Sigma offers a wide selection of protein visualization (staining) reagents. EZBlue™ and ProteoSilver™, designed specifically for proteomics, als...
Keywords: PAGE, Proteomics

Introduction to Western Blotting

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is termed as blotting. Proteins resolved on Sodium dodecyl sulfate - polyacrylamide gel electrophor...
Keywords: Buffers, Cell disruption, Detergents, Electroblotting, Electrophoresis, Gel electrophoresis, Homogenization, Immobilization, PAGE, Protein assay, Protein extraction, Sample preparations, Separation, Size-exclusion chromatography, Western blot

Peer-Reviewed Papers
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