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P7914 Sigma-Aldrich

Phthaldialdehyde Reagent

Solution Incomplete

Synonym: OPA

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Description

Application

Precolumn derivatization reagent for primary amines and amino acids. The fluorescent derivative can be detected by reverse-phase HPLC. The reaction requires OPA, primary amine and a sulfhydryl. In the presence of excess sulfhydryl, amines can be quantitated. In the presence of excess amine, sulfhydryls can be quantitated.

Useful for investigators requiring an alternate sulfhydryl moiety. Requires addition of desired sulfhydryl compound for measuring amines. Requires addition of amine to measure sulfhydryls.

Linkage

Same as P 0532, but contains no 2-mercaptoethanol.

Price and Availability

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN 1814 8 / PGII
WGK Germany 
2

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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