PHR1039 Sigma-Aldrich


Pharmaceutical Secondary Standard; Certified Reference Material

  • CAS Number 114-07-8

  • Empirical Formula (Hill Notation) C37H67NO13

  • Molecular Weight 733.93

  •  Beilstein Registry Number 75279

  •  EC Number 204-040-1

  •  MDL number MFCD00084654

  •  PubChem Substance ID 329823069



Related Categories Additional Standards, Analytical Standards, Analytical/Chromatography, Chromatography, Drugs & Metabolites,
grade   certified reference material
  pharmaceutical secondary standard
form   neat
solubility   ethanol: soluble50 mg/mL, clear, colorless to faintly yellow
format   neat
pharmacopeia traceability   traceable to BP 794
  traceable to PhEur E1305000
  traceable to USP 1242000



Erythromycin is an antibiotic produced by growth of certain strains of Streptomyces erythreus. This product is composed largely of erythromycin A with small amounts of erythromycins B and C and is recommended for concentration at 100 mg/L. Concentrations between 50 and 200 mg/L have also proven effective in controlling bacterial growth. Erythromycin has been used as a motilin receptor agonist, to block respiratory glycoconjugate secretion in human airways in vitro, and for selecting plasmid-cured and recombinant lactococcus lactis MG1363 strains.

Biochem/physiol Actions

Mode of Action: Erythromycin acts by inhibiting elongation at the transpeptidation step, specifically aminoacyl translocation from the A-site to P-site by binding to the 50s subunit of the bacterial 70s rRNA complex.

Antimicrobial Spectrum: This product acts against both gram-negative and gram-positive bacteria.


This product is stable in solution at 37°C for 3 days. Stock solutions should be stored at 2-8°C.

Preparation Note

This product is soluble in water at 2 mg/mL, with a 0.067% solution in water yielding a pH of 8.0-10.5. It is also soluble in ethanol at 50 mg/mL, yielding a clear, colorless to faint yellow solution. It is freely soluble in alcohol, acetone, chloroform, acetonitrile and ethyl acetate but forms salts with acids. All solutions should be protected from light.

Legal Information

Pharmaceutical secondary standards for application in quality control, provide pharma laboratories and manufacturers with a convenient and cost-effective alternative to the preparation of in-house working standards.

These secondary standards offer multi-traceability to the USP, EP (PhEur) and BP primary standards, where they are available. This product was designed, produced and verified for accuracy and stability in accordance with ISO/IEC 17025:2005 (AClass Cert AT-1467), ISO GUIDES 34:2009 (AClass Cert AR-1470).


To see an example of a Certificate of Analysis for this material enter LRAA8967 in the slot below. This is an example certificate only and may not be the lot that you receive.

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Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Protocols & Articles


LC/MS Analysis of Erythromycin Analogs on Ascentis® Express HILIC

From our library of Articles, Sigma-Aldrich presents LC/MS Analysis of Erythromycin Analogs on Ascentis® Express HILIC
Keywords: Antibiotics, Chromatography, High performance liquid chromatography, Pharmaceutical

Peer-Reviewed Papers


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