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S8625 Sigma-Aldrich

Sodium acetate trihydrate

ReagentPlus®, ≥99.0%

Synonym: Acetic acid sodium salt trihydrate



Related Categories Biochemicals and Reagents, Biological Buffers, Buffers A to Z, Essential Chemicals, Research Essentials,
grade   ReagentPlus®
assay   ≥99.0%
autoignition temp.   1112 °F
pKa (25 °C)   4.76 (acetic acid)


Other Notes

Enzyme substrate for acetate kinase (EC

A -D suffix exists for administrative purposes only.
All -D packages are 100% the same product, same quality, same specification as the package sizes previously sold without a -D.


1, 2 kg in poly bottle

250, 500 g in poly bottle

5 kg in poly drum

Legal Information

ReagentPlus is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability

Suggested Laboratory Gloves

Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.

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Safety & Documentation

Safety Information

WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles


Determination of Water Content in Sodium acetate trihydrate Using Karl Fischer Titration

Carboxylic acid salts can be straightforwardly analyzed provided the sample dissolves rapidly in the working medium. Dissolution is accelerated by the addition of formamide, titration at elevated tem...
Keywords: Titrations


Enzymatic Assay of Amyloglucosidase (EC

This procedure may be used for the determination of Amyloglucosidase activity using starch as the substrate. The Spectrophotometric Stop Rate Determination [Absorbance at 340 nm (A340), Light path = ...
Keywords: Enzyme activity, Extinction coefficient

Enzymatic Assay of Cellulase

3.12 Unit definition: One unit will liberate 1.0 μmole of glucose from cellulose in one hour at pH 5.0 at 37ºC (2 hour incubation time).
Keywords: Extinction coefficient

Enzymatic Assay of Chitosanase from Streptomyces Species

1. OBJECTIVE To standardize a procedure for determining the enzymatic activity of Chitosanase.
Keywords: Biochemistry, Enzyme activity, Microbiology

Enzymatic Assay of Choloylglycine Hydrolase

1. OBJECTIVE To standardize a procedure for the determination of the enzymatic assay of choloylglycine hydrolase.
Keywords: Enzyme activity

Enzymatic Assay of Deoxyribonuclease I (EC

1. OBJECTIVE To standardize a procedure for the enzymatic assay of Deoxyribonuclease I at Sigma-Aldrich St. Louis.

Enzymatic Assay of Glucose Oxidase

Derived from procedure SPGLUC01. Includes template updates to current SOP specification and incorporation of notes into the procedure. Refer to CR SOP-DEK ENZ 36.
Keywords: Extinction coefficient

Enzymatic Assay of Hyaluronidase (

The turbidimetric determination (% Transmittance at 600 nm, Light path = 1 cm) is based on the following reaction:
Keywords: Extinction coefficient

Enzymatic Assay of Invertase

2. SCOPE This procedure applies to all products that have specification for the enzymatic activity of Invertase at Sigma-Aldrich Saint Louis.
Keywords: Biochemistry, Enzyme activity, Enzymology

Enzymatic Assay of Protease Using Casein As a Substrate

1. OBJECTIVE To standardize a procedure for the enzymatic assay of Protease using Casein as a substrate at Sigma-Aldrich St. Louis.

Enzymatic Assay of Ribonuclease A (EC

This procedure may be used for determination of Ribonuclease A (RNase A) activity. The Spectrophotometric Stop Rate Determination [Absorbance at 300 nm (A300), Light path = 1 cm] is based on the foll...

Enzymatic Assay of Sulfatase

2. SCOPE This procedure applies to all products that have a specification for Sulfatase activity, except Sulfatase from Aerobacter aerogenes, Sigma-Aldrich Product Number S1629.
Keywords: Extinction coefficient

Enzymatic Assay of α-Glucosidase by the Modified Boehenger Procedure (EC

1. OBJECTIVE To standardize a procedure for the enzymatic assay of α-glucosidase by the modified Boehenger procedure at Sigma-Aldrich St. Louis.
Keywords: Enzyme activity, Extinction coefficient

Enzymatic Assay of β-AMYLASE (EC

1. OBJECTIVE To standardize a procedure for determining the enzymatic activity of β-Amylase.
Keywords: Enzyme activity, Enzymology

Suitability for the Qualitative Determination of Calcium with Phthalein Purple (O-Cresophthalein Complexone) using Stannous Chloride

1. OBJECTIVE To standardize a procedure to determine the suitability for the qualitative determination of calcium with phthalein purple at Sigma-Aldrich St. Louis.
Keywords: Absorption, Atomic absorption spectroscopy, Indicators, Inductively coupled plasma

Peer-Reviewed Papers


Set your institution to view full text papers.

Acetate Kinase of Bacteria (Acetokinase). I. Rose Meth. Enzymol. 1, 591, (1955)

Major complications, mortality, and resource utilization after open abdominal surgery: 0.9% saline compared to Plasma-Lyte. Shaw AD, Bagshaw SM, Goldstein SL, et al. Ann. Surg. 255(5), 821-9, (2012)


Randomized clinical trial of goal-directed fluid therapy within an enhanced recovery protocol for elective colectomy. Srinivasa S, Taylor MH, Singh PP, et al. Br. J. Surg. 100(1), 66-74, (2013)


Escherichia coli single-stranded DNA-binding protein: nanoESI-MS studies of salt-modulated subunit exchange and DNA binding transactions. Mason CE, Jergic S, Lo AT, et al. J. Am. Soc. Mass Spectrom. 24(2), 274-85, (2013)


Biodegradation of triclosan by a wastewater microorganism. Lee do G, Zhao F, Rezenom YH, et al. Water Res. 46(13), 4226-34, (2012)


Modeling of mixed-mode chromatography of peptides. Bernardi S, Gétaz D, Forrer N, et al. J. Chromatogr. A. 1283, 46-52, (2013)


Plasma-Lyte 148 vs 0.9% saline for fluid resuscitation in diabetic ketoacidosis. Chua HR, Venkatesh B, Stachowski E, et al. J. Crit. Care 27(2), 138-45, (2012)


Cognitive changes after saline or plasmalyte infusion in healthy volunteers: a multiple blinded, randomized, cross-over trial. Story DA, Lees L, Weinberg L, et al. Anesthesiology 119(3), 569-75, (2013)


A protocol for the 0.9% saline versus Plasma-Lyte 148 for intensive care fluid therapy (SPLIT) study. Reddy SK, Bailey MJ, Beasley RW, et al. Crit. Care Resusc. 16(4), 274-9, (2014)


A randomized, controlled, double-blind crossover study on the effects of 2-L infusions of 0.9% saline and plasma-lyte® 148 on renal blood flow velocity and renal cortical tissue perfusion in healthy volunteers. Chowdhury AH, Cox EF, Francis ST, et al. Ann. Surg. 256(1), 18-24, (2012)


Lipid droplet synthesis is limited by acetate availability in starchless mutant of Chlamydomonas reinhardtii. Ramanan R, Kim BH, Cho DH, et al. FEBS Lett. 587(4), 370-7, (2013)


Technical note: Bone DNA extraction and purification using silica-coated paramagnetic beads. Dukes MJ, Williams AL, Massey CM, et al. Am. J. Phys. Anthropol. 148(3), 473-82, (2012)


Biodegradation of aniline in an alkaline environment by a novel strain of the halophilic bacterium, Dietzia natronolimnaea JQ-AN. Jin Q, Hu Z, Jin Z, et al. Bioresour. Technol. 117, 148-54, (2012)


Impact of carbon source on nitrous oxide emission from anoxic/oxic biological nitrogen removal process and identification of its emission sources. Hu Z, Zhang J, Li S, et al. Environ. Sci. Pollut. Res. Int. 20(2), 1059-69, (2013)


Post separation adjustment of pH to enable the analysis of aminoglycoside antibiotics by microchip electrophoresis with amperometric detection. Ding Y, Bai L, Suo X, et al. Electrophoresis 33(21), 3245-53, (2012)


Corrosion inhibitors from expired drugs. Vaszilcsin N, Ordodi V, and Borza A Int. J. Pharm. 431(1-2), 241-4, (2012)


Self-interaction chromatography in pre-packed columns: a critical evaluation of self-interaction chromatography methodology to determine the second virial coefficient. Rakel N, Schleining K, Dismer F, et al. J. Chromatogr. A. 1293, 75-84, (2013)


Effects of different amine fluoride concentrations on enamel remineralization. Naumova EA, Niemann N, Aretz L, et al. J. Dent. 40(9), 750-5, (2012)


Short-chain fatty acids suppress lipopolysaccharide-induced production of nitric oxide and proinflammatory cytokines through inhibition of NF-κB pathway in RAW264.7 cells. Liu T, Li J, Liu Y, et al. Inflammation 35(5), 1676-84, (2012)


Characteristics and photo-responsive release property of liposome containing 7-acetoxy coumarin. Seo HJ, Kim JC. J. Nanosci. Nanotechnol. 11, 10262-10270, (2011)


Elemental diets may reduce the risk of aspiration pneumonia in bedridden gastrostomy-fed patients. Horiuchi A, Nakayama Y, Sakai R, et al. Am. J. Gastroenterol. 108(5), 804-10, (2013)


HPLC-fluorescence method for measurement of the uremic toxin indoxyl sulfate in plasma. Al Za'abi M, Ali B, and Al Toubi M J. Chromatogr. Sci. 51(1), 40-3, (2013)


Optimization of protein solution by a novel experimental design method using thermodynamic properties. Kim NA, An IB, Lee SY, et al. Arch. Pharm. Res. 35(9), 1609-19, (2012)


Effect of different buffer agents on in-vessel composting of food waste: performance analysis and comparative study. Li S, Huang GH, An CJ, et al. J. Environ. Sci. Health. A. Tox. Hazard. Subst. Environ. Eng. 48(7), 772-80, (2013)


Direct comparison of solid-phase gastric emptying times assessed by means of a carbon isotope-labeled sodium acetate breath test and technetium Tc 99m albumin colloid radioscintigraphy in healthy cats. Schmitz S, Götte B, Borsch C, et al. Am. J. Vet. Res. 75(7), 648-52, (2014)


HPAEC-PAD method for the analysis of alkaline hydrolyzates of Haemophilus influenzae type b capsular polysaccharide. de Haan A, van der Put RM, and Beurret M Biomed. Chromatogr. 27(9), 1137-42, (2013)


Stat5a increases lactation of dairy cow mammary gland epithelial cells cultured in vitro. Liu XF, Li M, Li QZ, et al. In Vitro Cell. Dev. Biol. Anim. 48(9), 554-61, (2012)


Effect of different biosynthetic precursors on the production of nargenicin A1 from metabolically engineered Nocardia sp. CS682. Koju D, Maharjan S, Dhakal D, et al. J. Microbiol. Biotechnol. 22(8), 1127-32, (2012)


Novel UV-spectrophotometric method for quantitative estimation of furazolidone using mixed hydrotropic agent. Jain N, Jain R, Jain DK, et al. Pak. J. Pharm. Sci. 26(1), 159-62, (2013)


Intravenous 0.9% saline and general surgical patients: a problem, not a solution. Lobo DN Ann. Surg. 255(5), 830-2, (2012)


Accumulation fatty acids of in Chlorella vulgaris under heterotrophic conditions in relation to activity of acetyl-CoA carboxylase, temperature, and co-immobilization with Azospirillum brasilense. Leyva LA, Bashan Y, Mendoza A, et al. Naturwissenschaften 101(10), 819-30, (2014)


Merck 14,8571

Beil. 2,IV,94

Fieser 1,1024 / Fieser 5,59

Aldrich MSDS 1, 10:A / Corp MSDS 1 (1), 15:D / RegBook 1 (1), 601:L / Structure Index 1, 86:C:4

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