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S9305 Sigma-Aldrich

SYBR® Green II RNA gel stain Green Alternative

10,000 × in DMSO



Legal Information

U.S. Patent No. 5,436,134. Licensed from Molecular Probes, Inc.

SYBR is a registered trademark of Life Technologies


On denaturing agarose/formaldehyde gels and polyacrylamide/urea gels, the sensitivity of SYBR Green II RNA gel stain is reduced, though still superior to that of ethidium bromide. Without any washing or destaining steps, SYBR Green II can detect as little as 1 ng RNA per band in agarose/formaldehyde gel or polyacrylamide/urea gel using 254 nm epi-illumination, and about 4 ng RNA per band using 300 nm trans-illumination. Staining agarose/ formaldehyde gels with SYBR Green II does not interfere with the transfer of RNA to membranes or subsequent hybridization in Northern blot analysis as long as 0.1%-0.3% SDS is included in prehybridization and hybridization buffers to remove the dye.

Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has inherently safer chemistry, compared to the standard use of ethidium bromide for staining. For more information see publication found in related content.

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Tissue Diagnostics

All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

NA 1993 / PGIII
WGK Germany 
Flash Point(F) 
188.6 °F
Flash Point(C) 
87 °C
Protocols & Articles


Nancy-520 for DNA Detection and Quantitation

Nancy-520 is a fluorescent stain for double stranded DNA (dsDNA) on agarose electrophoresis gels, with higher sensitivity than ethidium bromide and an easy, fast, and robust staining procedure. It is...
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BioFiles 2009, 4.1, 4.
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01494 Nancy-520, suitable for RT-PCR, ≥97.0% (HPCE)

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