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S9305 Sigma-Aldrich

SYBR® Green II RNA gel stain Green Alternative

10,000 × in DMSO

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Description

Legal Information

U.S. Patent No. 5,436,134. Licensed from Molecular Probes, Inc.

SYBR is a registered trademark of Life Technologies

Application

On denaturing agarose/formaldehyde gels and polyacrylamide/urea gels, the sensitivity of SYBR Green II RNA gel stain is reduced, though still superior to that of ethidium bromide. Without any washing or destaining steps, SYBR Green II can detect as little as 1 ng RNA per band in agarose/formaldehyde gel or polyacrylamide/urea gel using 254 nm epi-illumination, and about 4 ng RNA per band using 300 nm trans-illumination. Staining agarose/ formaldehyde gels with SYBR Green II does not interfere with the transfer of RNA to membranes or subsequent hybridization in Northern blot analysis as long as 0.1%-0.3% SDS is included in prehybridization and hybridization buffers to remove the dye.

Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has inherently safer chemistry, compared to the standard use of ethidium bromide for staining. For more information see publication found in related content.

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All labs need water
Safety & Documentation

Safety Information

RIDADR 
NA 1993 / PGIII
WGK Germany 
3
Flash Point(F) 
188.6 °F
Flash Point(C) 
87 °C
Protocols & Articles

Articles

Nancy-520 for DNA Detection and Quantitation

Nancy-520 is a fluorescent stain for double stranded DNA (dsDNA) on agarose electrophoresis gels, with higher sensitivity than ethidium bromide and an easy, fast, and robust staining procedure. It is...
Monika Baeumle
BioFiles 2009, 4.1, 4.
Keywords: Catalytic combustion detector, Electrophoresis

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Greener Alternatives Three Categories

A new icon has been added to identify our Greener alternative products. Products with this icon fulfill one of the three criteria.
Keywords: Alternative energy, Ceramics, Environmental, Green chemistry, Materials Science, Nanomaterials, PAGE

Peer-Reviewed Papers
15

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Description

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01494 Nancy-520, suitable for RT-PCR, ≥97.0% (HPCE)

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