|Related Categories||Biochemicals and Reagents, Core Bioreagents, Fluorescent Enzyme Substrates, Fluorescent Enzyme Substrates for Electrophoresis, Fluorescent Probes, Labels, Particles and Stains,|
|usage||1.0 mL sufficient for 100 mini-gels|
|concentration||10,000 × in DMSO|
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U.S. Patent No. 5,436,134. Licensed from Molecular Probes, Inc.
SYBR is a registered trademark of Life Technologies
A proprietary unsymmetrical cyanine dye which is an ultrasensitive stain for post-electrophoresis staining of dsDNA in agarose or polyacrylamide gels. SYBR Green I can also be used to detect ssDNA and RNA in denaturing agarose/formaldehyde and polyacrylamide/urea gels without any prewashing steps, although the sensitivity is lower (approximately 100 to 300 pg per band using 254 nm epi-illumination). SYBR Green II is recommended for ss-DNA and RNA.
SYBR Green I stain has been shown to be much less mutagenic than ethidium bromide in Ames tests. SYBR Green I is also a very sensitive stain for oligonucleotides, allowing for detection of as little as 1-2 ng of a synthetic 24-mer on a 5% polyacrylamide gel, which is 50-100 times greater sensitivity than obtained with ethidium bromide. Staining agarose gels with SYBR Green I does not interfere with the transfer of nucleic acids to membranes or subsequent hybridization in Southern or Northern blot analysis as long as 0.1%-0.3% SDS is included in prehybridization and hybridization buffers to remove the dye.
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|Personal Protective Equipment||Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)|
|Flash Point(F)||201.2 °F|
|Flash Point(C)||94 °C|
Biophys. J. 66, A159, (1994)
Biomed. Prod. 19, 68, (1994)
Dyes for in vitro detection of nucleic acids and proteins. BioProbes 20, 12-13, (1994)
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