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S9430 Sigma-Aldrich

SYBR® Green I nucleic acid gel stain

10,000 × in DMSO

Description

Frequently Asked Questions

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Legal Information

U.S. Patent No. 5,436,134. Licensed from Molecular Probes, Inc.

SYBR is a registered trademark of Life Technologies

Application

A proprietary unsymmetrical cyanine dye which is an ultrasensitive stain for post-electrophoresis staining of dsDNA in agarose or polyacrylamide gels. SYBR Green I can also be used to detect ssDNA and RNA in denaturing agarose/formaldehyde and polyacrylamide/urea gels without any prewashing steps, although the sensitivity is lower (approximately 100 to 300 pg per band using 254 nm epi-illumination). SYBR Green II is recommended for ss-DNA and RNA.
SYBR Green I stain has been shown to be much less mutagenic than ethidium bromide in Ames tests. SYBR Green I is also a very sensitive stain for oligonucleotides, allowing for detection of as little as 1-2 ng of a synthetic 24-mer on a 5% polyacrylamide gel, which is 50-100 times greater sensitivity than obtained with ethidium bromide. Staining agarose gels with SYBR Green I does not interfere with the transfer of nucleic acids to membranes or subsequent hybridization in Southern or Northern blot analysis as long as 0.1%-0.3% SDS is included in prehybridization and hybridization buffers to remove the dye.

Price and Availability

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10,000 × in DMSO

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in 10× TBE buffer, powder blend

≥97.0% (HPCE)

SYBR<SUP>®</SUP> Green II RNA gel stain

10,000 × in DMSO

SYBR<SUP>®</SUP> Green JumpStart<SUP>™</SUP> <I>Taq</I> ReadyMix<SUP>™</SUP>

for quantitative PCR, MgCI2 in buffer

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