SYBR® Green I nucleic acid gel stain
10,000 × in DMSO
DOWNLOAD MSDS (PDF)-
CAS Number 163795-75-3
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MDL number MFCD00284715
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Popular Documents: Datasheet (PDF)
Properties
| Related Categories | Biochemicals and Reagents, Core Bioreagents, Fluorescent Enzyme Substrates, Fluorescent Enzyme Substrates for Electrophoresis, Fluorescent Probes, Labels, Particles and Stains, |
| usage | 1.0 mL sufficient for 100 mini-gels |
| concentration | 10,000 × in DMSO |
| storage temp. | −20°C |
Description
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Legal Information
U.S. Patent No. 5,436,134. Licensed from Molecular Probes, Inc.
SYBR is a registered trademark of Life Technologies
Application
A proprietary unsymmetrical cyanine dye which is an ultrasensitive stain for post-electrophoresis staining of dsDNA in agarose or polyacrylamide gels. SYBR Green I can also be used to detect ssDNA and RNA in denaturing agarose/formaldehyde and polyacrylamide/urea gels without any prewashing steps, although the sensitivity is lower (approximately 100 to 300 pg per band using 254 nm epi-illumination). SYBR Green II is recommended for ss-DNA and RNA.
SYBR Green I stain has been shown to be much less mutagenic than ethidium bromide in Ames tests. SYBR Green I is also a very sensitive stain for oligonucleotides, allowing for detection of as little as 1-2 ng of a synthetic 24-mer on a 5% polyacrylamide gel, which is 50-100 times greater sensitivity than obtained with ethidium bromide. Staining agarose gels with SYBR Green I does not interfere with the transfer of nucleic acids to membranes or subsequent hybridization in Southern or Northern blot analysis as long as 0.1%-0.3% SDS is included in prehybridization and hybridization buffers to remove the dye.
Price and Availability
Nucleic Acid Electrophoresis Reagents
Find all the necessary reagents to perfomyour Nucleic Acid Electrophoresis research
in one place.
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≥97.0% (HPCE)
100 ×, solution
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