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S9430 Sigma-Aldrich

SYBR® Green I nucleic acid gel stain Green Alternative

10,000 × in DMSO

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Description

Frequently Asked Questions

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Legal Information

U.S. Patent No. 5,436,134. Licensed from Molecular Probes, Inc.

SYBR is a registered trademark of Life Technologies

Application

A proprietary unsymmetrical cyanine dye which is an ultrasensitive stain for post-electrophoresis staining of dsDNA in agarose or polyacrylamide gels. SYBR Green I can also be used to detect ssDNA and RNA in denaturing agarose/formaldehyde and polyacrylamide/urea gels without any prewashing steps, although the sensitivity is lower (approximately 100 to 300 pg per band using 254 nm epi-illumination). SYBR Green II is recommended for ss-DNA and RNA.
SYBR Green I stain has been shown to be much less mutagenic than ethidium bromide in Ames tests. SYBR Green I is also a very sensitive stain for oligonucleotides, allowing for detection of as little as 1-2 ng of a synthetic 24-mer on a 5% polyacrylamide gel, which is 50-100 times greater sensitivity than obtained with ethidium bromide. Staining agarose gels with SYBR Green I does not interfere with the transfer of nucleic acids to membranes or subsequent hybridization in Southern or Northern blot analysis as long as 0.1%-0.3% SDS is included in prehybridization and hybridization buffers to remove the dye.

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SYBR<SUP>®</SUP> Green II RNA gel stain

10,000 × in DMSO

SYBR<SUP>®</SUP> Green JumpStart<SUP>™</SUP> <I>Taq</I> ReadyMix<SUP>™</SUP>

for quantitative PCR, MgCI2 in buffer

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in 10× TBE buffer, powder blend

Nancy-520

suitable for RT-PRC, ≥97.0% (HPCE)

SYBR<SUP>®</SUP> Green II RNA gel stain

10,000 × in DMSO

SYBR<SUP>®</SUP> Green JumpStart<SUP>™</SUP> <I>Taq</I> ReadyMix<SUP>™</SUP>

for quantitative PCR, MgCI2 in buffer

Safety & Documentation

Safety Information

WGK Germany 
3
Flash Point(F) 
201.2 °F
Flash Point(C) 
94 °C

Protocols & Articles

Protocols

Complete Whole Transcriptome Amplification Kit WTA2 Protocol

Product Description Components Storage/Stability Procedure Troubleshooting Guide Frequently Asked Questions Materials References Publication

Hot Start dNTP protocol to reduce non-specific amplification

· How do Hot-Start dNTPs work? · Handling · Protocols for Taq DNA Polymerase—Standard PCR, Fast PCR, Multiplexed PCR and Real-Time PCR · Troubleshooting · Standard Thermal Cycling Conditions for Othe...
Keywords: AGE, Amplification, Buffers, Centrifugation, Degradations, Electrophoresis, Gel electrophoresis, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Purification, Size-exclusion chromatography

SeqPlex Enhanced DNA Amplification Kit Protocol

Product Description Workflow Reagents Provided Storage/Stability Procedure Appendix 1: Optional Primer Removal Assay Troubleshooting Guide FAQs References
Keywords: AGE, Absorption, Amplification, Capillary electrophoresis, Chromatin immunoprecipitation, Digestions, Electrophoresis, Gel electrophoresis, Genomics, Immunoprecipitation, Microarray Analysis, Molecular biology, Molecular probes, Nucleic acid annealing, Nucleic acid denaturation, Peptide synthesis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Polymerization reactions, Purification, Reductions, Sequencing, Sonication, Substitutions, Whole genome amplification

SeqPlex RNA Amplification Kit Protocol

The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms. Microgram quantit...

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01494 Nancy-520, suitable for RT-PRC, ≥97.0% (HPCE)

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