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S9938 Sigma-Aldrich

S-Gal®/LB Agar Blend, without IPTG

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Description

Components

Components (g/L)
Tryptone 10 g
Yeast extract 5 g
Sodium chloride 5 g
Agar 12 g
S-Gal 0.3 g
Ferric ammonium citrate 0.5 g

Application

S-Gal®/LB Agar Blend, without IPTG is designed for selection of recombinant bacterial clones with the lac+ phenotype.

Features and Benefits

S-Gal/LB Agar Blend, without IPTG is designed to be used in systems that do not require the inducer isopropyl β-D-thiogalactopyranoside (IPTG). It also gives the researcher the ability to control the level of inducer for systems that do require IPTG.

Other Notes

For researchers who don′t need (or want to control the amount of) inducer in their media.

Related Products: IPTG (Isopropyl β-D-thiogalactopyranoside, I6758), Ampicillin (A2804), Kanamycin (K0879), Chloramphenicol (C7795), Tetracycline (T8032)

Reconstitution

Suspend contents of one packet in 500 mL distilled or deionized water. Sterilize by autoclaving for 15 to 20 minutes at 121-124 °C. For microwaving, heat suspended mix until initial boiling. Mix well. Heat for short intervals with mixing until agar component is in solution. Do not allow boiling for extended periods of time. Antibiotics should be added following autoclaving or microwaving, after cooling to 48-52 °C.

Legal Information

S-GAL is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability


Pharma QC
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the environment, microbes have adapted to the habitats most suitable for thei...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

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