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SE100 Sigma-Aldrich

Silver Enhancer Kit

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Properties

Related Categories Cell Biology, Hematology and Histology, S, Stains & Dyes, A to Z, Stains and Dyes More...
usage   sufficient for ≥100 slides staining and fixing
storage temp.   2-8°C

Description

Features and Benefits

Colloidal gold labels are normally visible only at the electron microscope level. Silver Enhancer enlarges the gold colloid label by precipitation of metallic silver to give a high contrast signal visible under a light microscope. A darkroom is not necessary for this procedure, as the silver enhancer working solution is light-stable for 20-30 minutes under ordinary laboratory lighting.

Packaging

The Silver Enhancer kit consists of Solution A (silver salt), Solution B (initiator), Sodium Thiosulfate (fixer), and a Technical Bulletin describing their use.

Principle

Solution A and Solution B are mixed 1:1 immediately before use and applied to the slide holding the gold-labeled section. After 5-10 minutes, the slide is rinsed with distilled water, fixed for 2-3 minutes in a sodium thiosulfate solution, and rinsed again. The section is now ready for counterstaining and mounting as required.

Price and Availability


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Description

Product #

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Sodium thiosulfate pentahydrate, reagent grade, ≥99.5% 500 g SDS    
Silver enhancer solution A 100 mL SDS S5020
Silver enhancer solution B 100 mL SDS S5145
Safety & Documentation

Safety Information

Symbol 
GHS09  GHS09
Signal word 
Warning
Hazard statements 
RIDADR 
UN 3082 9 / PGIII

Documents

Certificate of Analysis

Protocols & Articles
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