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SE100 Sigma-Aldrich

Silver Enhancer Kit

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Properties

Related Categories Cell Biology, Hematology and Histology, S, Stains & Dyes, A to Z, Stains and Dyes More...
usage   sufficient for ≥100 slides staining and fixing
storage temp.   2-8°C

Description

Features and Benefits

Colloidal gold labels are normally visible only at the electron microscope level. Silver Enhancer enlarges the gold colloid label by precipitation of metallic silver to give a high contrast signal visible under a light microscope. A darkroom is not necessary for this procedure, as the silver enhancer working solution is light-stable for 20-30 minutes under ordinary laboratory lighting.

Packaging

The Silver Enhancer kit consists of Solution A (silver salt), Solution B (initiator), Sodium Thiosulfate (fixer), and a Technical Bulletin describing their use.

Principle

Solution A and Solution B are mixed 1:1 immediately before use and applied to the slide holding the gold-labeled section. After 5-10 minutes, the slide is rinsed with distilled water, fixed for 2-3 minutes in a sodium thiosulfate solution, and rinsed again. The section is now ready for counterstaining and mounting as required.

Price and Availability


All labs need water

Kit component also available separately

Description

Product #

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Sodium thiosulfate pentahydrate, reagent grade, ≥99.5% 500 g SDS    
Silver enhancer solution A 100 mL SDS S5020
Silver enhancer solution B 100 mL SDS S5145
Safety & Documentation

Safety Information

Symbol 
GHS09  GHS09
Signal word 
Warning
Hazard statements 
RIDADR 
UN 3082 9 / PGIII

Documents

Certificate of Analysis

Protocols & Articles
Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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