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W2261 Sigma-Aldrich

WL nutrient broth

for microbiology

Synonym: Wallerstein Laboratory Nutrient Broth

Purchase

Properties

Related Categories All Liquid Media/Broths, Candida, Escherichia coli & Coliforms, Lactobacillus, Media,
grade   for microbiology
shelf life   limited shelf life, expiry date on the label
final pH   5.5±0.2 (25 °C)
suitability   selective for Candida
  selective for Escherichia coli and Coliforms
  selective for Lactobacillus
  selective for Pichia
  selective for Proteus
  selective for Saccharomyces
  selective for Zygosaccharomyces

Description

Components

Ingredients (g/L)
Casein enzymatic hydrolysate, 5.00
Yeast extract, 4.00
Dextrose, 50.00
Monopotassium phosphate, 0.55
Potassium chloride, 0.425
Calcium chloride, 0.125
Manganese sulfate, 0.125
Ferric chloride, 0.0025
Manganese sulfate, 0.0025
Bromocresol green, 0.022

Application

Recommended for the cultivation of bacteria encountered in breweries and industrial fermentations.

Preparation Note

Supplement: optional:1% solution sodium bicarbonate (Cat. No. 71627)

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Culture Method for Quality Control of Beer

Traditional methods are based morphology, staining methods, enzyme reactions (metabolism) and diverse media. One of the first steps is the filtration of the beer followed by an incubation of the filt...
Jvo Siegrist
Microbiology Focus Edition 1.4
Keywords: Fermentation, Filtration, Magnetic resonance spectroscopy, Metabolism, Microbiology

Microbiological Media and Detection

Molecular biology-based methods, like PCR, can be used for lactobacilli detection. However, they are often quite expensive. Sigma-Aldrich provides a revolutionary molecular biology method that is rap...
Chloe McClanahan
BioFiles Edition 6.2
Keywords: Amplification, Anaerobic, Antimicrobials, Magnetic resonance spectroscopy, Molecular biology, Nucleic acid hybridization, Polymerase chain reaction, Reductions

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the environment, microbes have adapted to the habitats most suitable for thei...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Probiotics and Human Health

At the beginning of the 20th century, probiotics were thought to have a beneficial effect to the host by improving the intestinal microbial balance. Through his studies and observations, the Russian ...
Chloe McClanahan
BioFiles Edition 6.2
Keywords: AGE, Absorption, Adhesion, Amplification, Anaerobic, Antimicrobials, Apoptosis, Cancer, Capillary electrophoresis, Carcinogens, Cell culture, Cell proliferation, Chromatography, Coagulation, Detoxification, Diagnostic, Diseases, Enzyme activity, Fermentation, Gas chromatography, Gastrointestinal, Genomics, High performance liquid chromatography, Immunology, Inflammation, Magnetic resonance spectroscopy, Metabolism, Metabolites, Microbiology, Molecular biology, Nucleic acid hybridization, Pharmaceutical, Phosphorylations, Polymerase chain reaction, Polymerase chain reaction - quantitative, Prebiotics, Probiotics, Reductions, Respiratory, Sequencing, Thin layer chromatography, Titrations

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