X114 Sigma-Aldrich

Triton X-114

laboratory grade

Synonym: (1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol, Polyethylene glycol tert-octylphenyl ether



Related Categories Biochemicals and Reagents, Cell Culture, Cell Lysis and Protein Extraction Reagents, Core Bioreagents, Detergents,
grade   laboratory grade
description   non-ionic
mol wt   average mol wt 537
composition   t-Oct-C6H4-(OCH2CH2)xOH, x = 7-8
refractive index   n20/D 1.488(lit.)
CMC   0.2 mM(20-25°C)
transition temp   pour point ~−9 °C
  cloud point 23 °C
  cloud point 23 °C
density   1.058 g/mL at 25 °C(lit.)
HLB   12.4



A non-ionic detergent with a low cloud point (23 °C) enabling protein solubilization with phase-partitioning of hydrophilic from amphiphilic proteins


1 L in poly bottle

100, 500 mL in poly bottle

5 gal in poly drum

1 gal in poly bottle

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Price and Availability

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Safety & Documentation

Safety Information

Signal word 
Hazard statements 
Precautionary statements 
UN 3082 9 / PGIII
WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles


GPI Anchored Glycoproteins

Glycosylphosphatidylinisotol (GPI) anchored proteins have been identified throughout a broad range of eukaryotic species ranging from humans to insects, yeasts, bacteria, and fungi, suggesting they a...
Glycobiology Analysis Manual, 2nd Edition
Keywords: Adhesion, Cancer, Glycosylations, Transduction

Peer-Reviewed Papers


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Phase separation of integral membrane proteins in Triton X-114 solution. Bordier C., J. Biol. Chem. 256, 1604-1607, (1981)


Isolation of integral membrane proteins by phase partitioning with Triton X-114. Brusca, J.S. and Radolf, J.D Meth. Enzymol. 228, 182-93, (1994)


Lipopolysaccharide is a frequent contaminant of plasmid DNA preparations and can be toxic to primary human cells in the presence of adenovirus. Cotten, M, et al. Gene Ther. 1, 239-246, (1994)


Cloud point sample clean-up and capillary zone electrophoresis with field enhanced sample injection and micelle to solvent stacking for the analysis of herbicides in milk. Kukusamude C, Srijaranai S, Kato M, et al. J. Chromatogr. A 1351, 110-4, (2014)


Vortex-assisted surfactant-enhanced emulsification microextraction based on solidification of floating organic drop followed by electrothermal atomic absorption spectrometry for speciation of antimony (ΙΙΙ, V). Eftekhari M, Chamsaz M, Arbab-Zavar MH, et al. Environ. Monit. Assess. 187(1), 4129, (2015)


Enhancement of anti-cholinesterase activity of Zingiber cassumunar essential oil using a microemulsion technique. Okonogi S and Chaiyana W Drug Discov. Ther. 6(5), 249-55, (2012)


Methods for chromatographic removal of endotoxin. Lowe AJ, Bardliving CL, and Batt CA Methods Mol. Biol. 899, 265-75, (2012)


Assessment of gelatinases (MMP-2 and MMP-9) by gelatin zymography. Toth M, Sohail A, and Fridman R Methods Mol. Biol. 878, 121-35, (2012)


Determination of cadmium(II), cobalt(II), nickel(II), lead(II), zinc(II), and copper(II) in water samples using dual-cloud point extraction and inductively coupled plasma emission spectrometry. Zhao L, Zhong S, Fang K, et al. J. Hazard. Mater. 239-240, 206-12, (2012)


Species selective preconcentration and quantification of gold nanoparticles using cloud point extraction and electrothermal atomic absorption spectrometry. Hartmann G and Schuster M Anal. Chim. Acta 761, 27-33, (2013)


Cloud point-dispersive μ-solid phase extraction of hydrophobic organic compounds onto highly hydrophobic core-shell Fe₂O₃@C magnetic nanoparticles. Giokas DL, Zhu Q, Pan Q, et al. J. Chromatogr. A 1251, 33-9, (2012)


Cloud point extraction of copper, lead, cadmium, and iron using 2,6-diamino-4-phenyl-1,3,5-triazine and nonionic surfactant, and their flame atomic absorption spectrometric determination in water and canned food samples. Citak D and Tuzen M J. AOAC Int. 95(4), 1170-5, (2012)


Cloud point extraction and spectrophotometric determination of mercury species at trace levels in environmental samples. Ulusoy Hİ, Gürkan R, and Ulusoy S Talanta 88, 516-23, (2012)


Protective efficacy of a bacterially produced modular capsomere presenting M2e from influenza: extending the potential of broadly cross-protecting epitopes. Wibowo N, Hughes FK, Fairmaid EJ, et al. Vaccine 32(29), 3651-5, (2014)


Identification of the major proteins of an immune modulating fraction from adult Fasciola hepatica released by Nonidet P40. Morphew RM, Hamilton CM, Wright HA, et al. Vet. Parasitol. 191(3-4), 379-85, (2013)


Simultaneous preconcentration of copper and mercury in water samples by cloud point extraction and their determination by inductively coupled plasma atomic emission spectrometry. Shoaee H, Roshdi M, Khanlarzadeh N, et al. Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 98, 70-5, (2012)


Immunoproteomics of Brucella abortus reveals differential antibody profiles between S19-vaccinated and naturally infected cattle. Pajuaba AC, Silva DA, Almeida KC, et al. Proteomics 12(6), 820-31, (2012)


Novel coupling of surfactant assisted emulsification dispersive liquid-liquid microextraction with spectrophotometric determination for ultra trace nickel. Deng Q, Chen M, Kong L, et al. Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 104, 64-9, (2013)


The macrophage migration inhibitory factor homolog of Entamoeba histolytica binds to and immunomodulates host macrophages. Moonah SN, Abhyankar MM, Haque R, et al. Infect. Immun. 82(9), 3523-30, (2014)


Effect of the GPI anchor of human Thy-1 on antibody recognition and function. Bradley JE, Chan JM, and Hagood JS Lab. Invest. 93(3), 365-74, (2013)


Phase-locked mutants of Mycoplasma agalactiae: defining the molecular switch of high-frequency Vpma antigenic variation. Chopra-Dewasthaly R Mol. Microbiol. 67(6), 1196-210, (2008)


Cloud point extraction and flame atomic absorption spectrometry determination of lead (II) in environmental and food samples. Soylak M, Yilmaz E, Ghaedi M, et al. J. AOAC Int. 95(6), 1797-802, (2012)


HPTLC method for the simultaneous determination of four indole alkaloids in Rauwolfia tetraphylla: a study of organic/green solvent and continuous/pulse sonication. Gupta S, Shanker K, and Srivastava SK J. Pharm. Biomed. Anal. 66, 33-9, (2012)


Removing endotoxin from plasmid samples by Triton X-114 isothermal extraction. Ma R, Zhao J, Du HC, et al. Anal. Biochem. 424(2), 124-6, (2012)


Oligomerization of ZFYVE27 (Protrudin) is necessary to promote neurite extension. Pantakani DV, Czyzewska MM, Sikorska A, et al. PLoS ONE 6(12), e29584, (2011)


Study on the determination of heavy metals in water samples with ultrasound-assisted dispersive liquid-liquid microextraction prior to FAAS. Li Z, Yu G, Song J, et al. Water Sci. Technol. 67(2), 247-53, (2013)


Improved quantification of HIV-1-infected CD4+ T cells using an optimised method of intracellular HIV-1 gag p24 antigen detection. Yang H, Yorke E, Hancock G, et al. J. Immunol. Methods 391(1-2), 174-8, (2013)


On a fully closed state of native human type-1 VDAC enriched in Nonidet P40. Thinnes FP and Burckhardt G Mol. Genet. Metab. 107(3), 632-3, (2012)


LC/MS method using cloud point extraction for the determination of permitted and illegal food colors in liquid, semiliquid, and solid food matrixes: single-laboratory validation. Ates E, Mittendorf K, and Senyuva H J. AOAC Int. 94(6), 1853-62, (2011)


Quantification of arsenic in dialysate solution and scalp hair samples of kidney failure patients by cloud point extraction and electrothermal atomic absorption spectroscopy. Baig JA, Kazi TG, Arain MB, et al. J. AOAC Int. 95(6), 1755-60, (2012)


Preparation and characterization of uniform particles of flufenamic acid and its calcium and barium salts. Mohamed AA and Matijević E J. Colloid. Interface Sci. 381(1), 198-201, (2012)


Extraction of the antimicrobial peptide cerein 8A by aqueous two-phase systems and aqueous two-phase micellar systems. Lappe R, Sant'anna V, and Brandelli A Nat. Prod. Res. 26(23), 2259-65, (2012)


A green method using a micellar system for determination of andrographolide and dehydroandrographolide in human plasma. Zhao Q, Ding J, Jin H, et al. J. Chromatogr. Sci. 51(4), 341-8, (2013)


Merck 13,6793

Corp MSDS 1 (2), 3545:D / FT-NMR 1 (1), 347:C / RegBook 1 (1), 1351:A / Structure Index 1, 31:D:8 / Structure Index 1, 209:B:6

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