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01632 Sigma

Atto 520-Biotin

BioReagent, suitable for fluorescence, ≥95.0% (HPCE)

Synonym: Biotin-Atto 520

  • Empirical Formula (Hill Notation) C37H53ClN6O8S

  • Molecular Weight 777.37

  •  MDL number MFCD04040671

  •  PubChem Substance ID 329747336

  •  eCl@ss 34058011

  • Popular Documents:  Datasheet (PDF)  

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Properties

Related Categories Biochemicals and Reagents, Biotin Conjugates, Fluorescent Conjugates, Fluorescent Probes, Labels, Particles and Stains
InChI Key   OCYRRKMPTKPPHX-QITHAKLPSA-N
product line   BioReagent
assay   ≥95.0% (HPCE)
solubility   DMF: soluble
  ethanol: soluble
  methanol: soluble
fluorescence   λex 520 nm; λem 540 nm in 0.1 M phosphate pH 7.0
suitability   suitable for fluorescence
storage temp.   2-8°C

Description

General description

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Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Application

Atto 520 is a fluorescent label with high molecular absorption (110.000) and quantum yield (0.90) as well as sufficient stoke’s shift (excitation maximum 520 nm, emission maximum 542 nm). It does nearly not show cis-trans-isomerisation, which limits brightness and reproducibility for many other dyes. Due to an insignificant triplet formation rate it is well suited for single molecule detection applications. Can be utilized in applications such as ELISA or immuno-histochemistry, in situ hybridization, flow cytometry and others, to identify streptavidin, avidin, or extravidin-conjugates. Atto-520 fluorescent-labeled biotin can be loaded onto magnetic beads coated with streptavidin through specific ligand–receptor interactions.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Atto Dyes for Superior Fluorescent Imaging Technology

Activated fluorescent dyes are routinely used to tag proteins, nucleic acids, and other biomolecules for use in life science applications including fluorescence microscopy, flow cytometry, fluorescen...
BioFiles 2011, 6.3, 5.
Keywords: Absorption, Applications, Bacterial conjugations, Confocal microscopy, Degradations, Enzyme Assays, Extinction coefficient, Flow cytometry, In Situ hybridization, Infrared spectroscopy, Isomerizations, Microarray Analysis, Microscopy, Molecular probes, Nucleic acid hybridization, Protocols, Semiconductor, Separation, Spectroscopy, Support

Peer-Reviewed Papers
15

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