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01815 Sigma

Actinomycin D

for fluorescence, ≥90% (HPLC), from Streptomyces sp.

Synonym: Actinomycin C1, Actinomycin IV, Dactinomycin

  • CAS Number 50-76-0

  • Empirical Formula (Hill Notation) C62H86N12O16

  • Molecular Weight 1255.42

  •  Beilstein Registry Number 605235

  •  EC Number 200-063-6

  •  MDL number MFCD00005033

  •  PubChem Substance ID 24845202

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Properties

Related Categories A - K, Antibacterial, Antibiotics, Antibiotics A to Z, Antibiotics A-F,
biological source   from Streptomyces sp.
grade   for fluorescence
InChI Key   RJURFGZVJUQBHK-IIXSONLDSA-N
assay   ≥90% (HPLC)
color   red, powder
mp   251-253
solubility   H2O: soluble0.5 mg/mL
  DMSO: soluble1 mg/mL
  acetone: soluble10 mg/mL
  acetonitrile: soluble10 mg/mL
fluorescence   λex 420; λem 443 in ethanol
Mode of action   DNA synthesis | interferes
antibiotic activity spectrum   neoplastics
storage temp.   2-8°C

Description

Application

Actinomycin D is an antineoplastic antibiotic that inhibits cell proliferation with wide-ranging applications ranging from a selection agent in cell culture, use in studies of suppressing HIV-replication and programmed cell death of PC12 cells, and as an antibiotic in treatment of various malignant neoplasms, including Wilm′s tumour and the sarcomas. Recommended for us in cell culture applications at 1μg/mL.

Caution

This product is hygroscopic and light-sensitive. If stored protected from light and moisture at 2-8°C, it remains unchanged, by HPLC testing, for at least 15 months. Unused dilute solutions of Actinomycin D are extremely sensitive to light and tend to adsorb to plastic and glass and should be discarded. However, frozen aliquots of concentrated stock solution are stable for at least a month.

Packaging

Product is packaged in a bottomless glass bottle with the contents inside an inserted fused cone.

Preparation Note

Actinomycin D is sold as red, shiny crystals and is soluble in acetonitrilie or acetone at 10 mg/mL and in DMSO at a minimum of 1 mg/mL. Some references also show slight solubility in water of 0.5 mg/mL.

Biochem/physiol Actions

Actinomycin D inhibits the proliferation of cells by forming a stable complex with double-stranded DNA, inhibiting DNA-primed RNA synthesis and causing single-stranded breaks in DNA. It has been shown to be an inhibitor of the minus-strand transfer step in reverse transcriptase.

Price and Availability

Safety & Documentation

Safety Information

Symbol 
GHS06  GHS06
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 3462 6.1 / PGII
WGK Germany 
3
RTECS 
AU1575000
Protocols & Articles
Peer-Reviewed Papers
15

References

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