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02065 Sigma

Adenosine 5′-triphosphate, immobilized on Agarose 4B

for affinity chromatography, powder (lyophilized)

Synonym: 5′-ATP-agarose 4B

  •  PubChem Substance ID 24845221

  •  eCl@ss 32160414

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Properties

grade   for affinity chromatography
form   powder (lyophilized)
contains   60% lactose as stabilizer
capacity   ≥1 μmol/mL, packed gel capacity (5′-ATP)(bound by a C6-spacer to C-8 of ATP)
storage temp.   −20°C

Description

General description

The lactose-stabilizer must be removed prior to use by washing the gel onto filter with water or buffer.

Other Notes

Purification of cofactor-dependent enzymes by affinity chromatography.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Application

Adenosine 5′-triphosphate, immobilized on Agarose 4B (5′-ATP-agarose 4B) is intended for use in affinity chromatography. 5′-ATP-agarose 4B has been shown to bind enzymes with affinity to 5′-ATP. 5′-ATP-agarose 4B may be considered for used with enzymes that bind to other ATP-agarose and sepharose affinity media or beads.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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