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02744 Sigma

Atto 655-Streptavidin

BioReagent, suitable for fluorescence

Synonym: Streptavidin-Atto 655

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Properties

Related Categories Biochemicals and Reagents, Fluorescent Conjugates, Fluorescent Probes, Labels, Particles and Stains, Streptavidin/Avidin Conjugates
product line   BioReagent
solubility   DMF: soluble
  DMSO: soluble
  acetonitrile: soluble
fluorescence   λex 655 nm; λem 680 nm in 0.1 M phosphate pH 7.0
suitability   suitable for fluorescence
  suitable for gel electrophoresis
storage temp.   −20°C

Description

Analysis Note

free of unconjugated dye

General description

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Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Application

Atto 655 is a fluorescent label with high molecular absorption (125.000) and quantum yield (0.30), as well as sufficient stoke’s shift (excitation maximum 663 nm, emission maximum 684 nm). There is very little cis-trans-isomerisation, which limits brightness and reproducibility for many other dyes. Atto 655 has been coupled to streptavidin to enable various types of assays based on the specific affinity of biotin to streptavidin. Streptavidin conjugates are used as secondary detection reagents in histochemical applications, flow cytometry, microarrays, blot analysis and immunoassays.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Atto Dyes for Superior Fluorescent Imaging Technology

Activated fluorescent dyes are routinely used to tag proteins, nucleic acids, and other biomolecules for use in life science applications including fluorescence microscopy, flow cytometry, fluorescen...
BioFiles 2011, 6.3, 5.
Keywords: Absorption, Applications, Bacterial conjugations, Confocal microscopy, Degradations, Enzyme Assays, Extinction coefficient, Flow cytometry, In Situ hybridization, Infrared spectroscopy, Isomerizations, Microarray Analysis, Microscopy, Molecular probes, Nucleic acid hybridization, Protocols, Semiconductor, Separation, Spectroscopy, Support

Fluorescent Labeling of Peptides

Labeling peptides with fluorescent dyes or other labels provides powerful tools for the investigation of biological relevant interactions like receptor-ligand-binding,1-3 protein structures,4-6 and e...
ChemFiles Volume 5 Article 12
Keywords: Alzheimer Disease, Biochemistry, Electrophoresis, Enzyme activity, Immobilization, Ligands, Methods, Microscopy, Peptide synthesis, Separation, Solid phase peptide synthesis, Solvents, Spectroscopy, Tools

Peer-Reviewed Papers
15

References

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