EMAIL THIS PAGE TO A FRIEND
03285 Sigma

Cell Counting Kit

sufficient for 500 tests

Purchase

Description

Application

The Cell Counting Kit (CCK) is utilized for the fluorometric detection of living cell numbers. The amount of a fluorescent dye, calcein, produced from calcein-AM (3′,6′-Di(O-acetyl)-2′,7′-bis[N,N-bis-(carboxymethyl) aminomethyl]-fluorescein, tetraacetoxymethyl ester) by esterases in cells is directly proportional to the number of viable cells in a culture medium. Since calcein-AM is highly lypohilic because of the acetoxymethy groups in the molecule, it can rapidly permeate into the cytoplasm through the cell membrane. The CCK assay does not require any radioisotopes (such as in the [3H]- thymidine incorporated assay) or a solubilization procedure (such as in the MTT assay). Therefore, it allows the users to obtain highly reproducible and accurate cell proliferation assay results.

Price and Availability


All labs need water

Scepter 2.0

Kit component only

Description

Product #

Add to Cart

Calcein-AM reagent SDS    
DPBS SDS    
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Cell Viability and Proliferation Assays

Assays to measure cellular proliferation, cell viability, and cytotoxicity are commonly used to monitor the response and health of cells in culture after treatment with various stimuli. The proper ch...
Article Based on
BioFiles v6 n5, 17–21
Keywords: Cancer, Cell proliferation, Click chemistry, Enzyme-linked immunosorbent assay, Flow cytometry, Glycolysis, Immunocytochemistry, Immunohistochemistry, Microscopy, Nucleic acid denaturation, Reductions, Solvents, Western blot

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?