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03449 Sigma

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride

≥99.0% (AT)

Synonym: N-Ethyl-N′-(3-dimethylaminopropyl)carbodiimide hydrochloride, EDAC, EDC hydrochloride, EDC, WSC hydrochloride

  • CAS Number 25952-53-8

  • Empirical Formula (Hill Notation) C8H17N3 · HCl

  • Molecular Weight 191.70

  •  Beilstein Registry Number 5764110

  •  EC Number 247-361-2

  •  MDL number MFCD00012503

  •  PubChem Substance ID 329747707

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Properties

Related Categories Crosslinking, Heterobifunctional Cross-Linking Reagents, Molecular Biology, Protein Modification, Protein Structural Analysis,
InChI Key   FPQQSJJWHUJYPU-UHFFFAOYSA-N
assay   ≥99.0% (AT)
mp   110-115 °C(lit.)
  112-116 °C
solubility   H2O: soluble0.2 g/L
storage temp.   −20°C

Description

Other Notes

Water soluble peptide coupling reagent; Modification of carboxyl groups in proteins, review; Synthesis of esters.

Application

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride was used for amine coupling reaction during self-assembly of the tethered bilayer lipid membrane. It was also used for derivatizing antibody against COX5b to carboxylated polystyrene microspheres.

Biochem/physiol Actions

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide is a water soluble condensing reagent. EDAC is generally utilized as a carboxyl activating agent for amide bonding with primary amines. Additionally, it will react with phosphate groups. It has been utilized in peptide synthesis, crosslinking proteins to nucleic acids as well as preparation of immunoconjugates.

Water soluble condensing reagent. EDAC is generally utilized as a carboxyl activating agent for amide bonding with primary amines. In addition, it will react with phosphate groups. EDAC has been used in peptide synthesis; crosslinking proteins to nucleic acids; and preparation of immunoconjugates as examples. Typically, EDAC is utilized in the pH range 4.0-6.0 without buffers. In particular, amine and carboxylate buffers should be avoided.

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Amplified Detection
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
FF2200000
Protocols & Articles

Articles

Carbodiimides

Carbodiimide-mediated peptide coupling remains to the most frequently used technique. As a major advantage, carbodiimides do not require prior activation of the carboxylic acid. Dicyclohexylcarbodiim...
Matthias Junkers
Aldrich ChemFiles 2007, 7.2, 5.
Keywords: Cyclizations, Peptide synthesis, Solid phase peptide synthesis

Graphene Field Effect Transistors for Biological and Chemical Sensors

Victoria Tsai* and Bruce Willner Graphene Frontiers, Philadelphia, PA 19104, USA *Email: victoria@graphenefrontiers.com
Keywords: Adhesion, Chemical vapor deposition, Chromatin immunoprecipitation, Deposition, Drug discovery, Environmental, Evaporation, Ion-exchange chromatography, Nanomaterials, Nanotubes, Semiconductor, Separation

Peer-Reviewed Papers
15

References

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