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03978 Sigma

Methylene Blue solution

for microscopy, concentrate according to Ehrlich, concentrated, aqueous solution

Synonym: Ehrlich’s reagent III, Loeffler’s Methylene Blue, Methylene blue stain

  • CAS Number 61-73-4

  • Empirical Formula (Hill Notation) C16H18ClN3S

  • Molecular Weight 319.85

  •  Beilstein Registry Number 3641570

  •  MDL number MFCD00012111

  •  PubChem Substance ID 329747853

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Properties

Related Categories Cell Biology, Hematology and Histology, M, Stains & Dyes, A to Z, Stains and Dyes More...
grade   for microscopy
InChI Key   CXKWCBBOMKCUKX-UHFFFAOYSA-M
quality   concentrated, aqueous solution
shelf life   ~3 yr
concentration   1.5% (1.5 g/ 100 mL)
density   1.0 g/mL at 20 °C

Description

Application

Methylene blue is used to stain animal cells to make their nuclei more observable. It is a component of staining media such as Wright’s stain and Jenner’s stain. Methylene blue is used to stain blood films/smears used in cytology and to stain RNA or DNA for viewing under the microscope or on hybridization membranes.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles
Peer-Reviewed Papers
15

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