Properties
| Related Categories | Cell Culture, ECACC Cell Lines, S-Z More... |
| biological source | Ovary from insect |
| growth mode | Adherent |
| morphology | Spherical |
| shipped in | dry ice |
| storage temp. | −196°C |
Description
Application
Protein expression using the Baculovirus vector system
General description
For complete product information, please see Sf21
Culture Medium
SF90011 + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 5x10,000 cells/cm2; 28 C. Gently resuspend cells in old culture medium by dislodging the cells by pipetting, or gentle agitation, centrifuge suspension and resuspend cell pellet in fresh medium.
Cell Line Description
Derived from pupal ovarian tissue of spodoptera frugiperda. The cells are highly susceptible to Baculovirus infection and are used in the production of protein products genetically manipulated into Baculovirus vector systems. They were derived at the USDA Insect Pathology Laboratory from a cell line originating from pupal ovarian tissue of the fall army worm, Spodoptera frugiperda. Sf21 cells have greater range in size and have a doubling time of 24 hr compared to 48-72 hr for Sf9 cells.
Price and Availability
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