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05066 Sigma

Agarose

High EEO, for molecular biology

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Properties

Related Categories Agarose, Core Bioreagents, Molecular Biology, Nucleic Acid Electrophoresis, Research Essentials More...
grade   for molecular biology
impurities   DNases, none detected
  RNases, none detected
  phosphatases, none detected
  proteases, none detected
ign. residue   ≤1%
loss   ≤10% loss on drying
EEO   0.23-0.27
transition temp   gel point 34-37 °C (1.5% solution)
gel strength   ≥1500 g/cm2 (1.5% gel)
anion traces   chloride (Cl-): ≤3000 mg/kg
  sulfate (SO42-): ≤6000 mg/kg
cation traces   Ca: ≤500 mg/kg
  Cd: ≤10 mg/kg
  Co: ≤10 mg/kg
  Cr: ≤10 mg/kg
  Cu: ≤10 mg/kg
  Fe: ≤10 mg/kg
  K: ≤500 mg/kg
  Mg: ≤10 mg/kg
  Mn: ≤10 mg/kg
  Na: ≤5000 mg/kg
  Ni: ≤10 mg/kg
  Pb: ≤10 mg/kg
  Zn: ≤10 mg/kg

Description

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Application

Agarose is used for preparative and analytical separation of nucleic acids. It can be used for single cell gel electrophoresis assay. It can also be used for southern blotting. It can also be used for assessing transepithelial ionic fluxes from cultured neonatal rat semicircular canal epithelium.

Biochem/physiol Actions

Agarose is a polysaccharide used for resolving DNA and RNA fragments from 500 - 20,000 bp. It provides strong gel structure which assists in better handling and less breakage. It can also reexpress the differentiated collagen phenotype from dedifferentiated chondrocytes during agarose gel culture.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles
Peer-Reviewed Papers
15

References

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