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05073 Sigma

Agarose

for molecular biology, low gelling temperature

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Properties

Related Categories Agarose, Core Bioreagents, Molecular Biology, Nucleic Acid Electrophoresis, Research Essentials More...
grade   for molecular biology
quality   low gelling temperature
impurities   DNases, none detected
  RNases, none detected
ign. residue   ≤1%
loss   ≤10% loss on drying
EEO   0.06-0.14
transition temp   gel point 31-39 °C (4% solution)
gel strength   >1200 g/cm2 (4% solution)
anion traces   sulfate (SO42-): ≤1500 mg/kg

Description

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Application

For preparative and analytical separation of nucleic acids ≤1000 bp

Price and Availability

Safety & Documentation

Safety Information

WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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