05470 Sigma

Bovine serum albumin

lyophilized powder, crystallized, ≥98.0% (GE)

Synonym: Albumin bovine serum, BSA, Bovine Serum Albumin, Bovine albumin



Related Categories Albumin, Biochemicals and Reagents, Bovine Serum Albumins, Ethanol Fractionated, Proteins and Derivatives More...
assay   ≥98.0% (GE)
form   lyophilized powder
mol wt   mol wt ~66 kDa
packaging   glass bottle of
pH   4.8-7.5
solubility   H2O: slightly soluble
anion traces   chloride (Cl-): ≤2000 ppm
  sulfate (SO42-): ≤1000 ppm
cation traces   Ca: ≤500 ppm
  Cd: ≤5 ppm
  Co: ≤5 ppm
  Cr: ≤100 ppm
  Cu: ≤20 ppm
  Fe: ≤20 ppm
  K: ≤50 ppm
  Mg: ≤500 ppm
  Mn: ≤5 ppm
  Na: ≤7000 ppm
  Ni: ≤5 ppm
  Pb: ≤5 ppm
  Zn: ≤10 ppm
storage temp.   2-8°C


Other Notes


Sales restrictions may apply


Bottomless glass bottle. Contents are inside inserted fused cone.

Preparation Note

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.


Albumin from bovine serum can be used for Electron and Immunoelectron microscopy. It can also be used for isolating and culturing neonatal rat cardiac ventricular myocytes.

Biochem/physiol Actions

Bovine serum albumin also refers as BSA or Fraction V is a protein isolated from cows. BSA can block vacant binding sites in enzyme-linked immunosorbent assay (ELISA) in both poly-l-lysine (PLL)-treated and non-treated microwells. It can also triggers the insulin dependent diabetes mellitus.

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Price and Availability

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles


HPLC Analysis of Proteins on Zenix® SEC-300, 30 cm x 7.8 mm I.D., 3 μm

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on Zenix® SEC-300, 30 cm x 7.8 mm I.D., 3 μm
Keywords: Chromatography, High performance liquid chromatography, Separation, Size-exclusion chromatography

Peer-Reviewed Papers


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