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06966 Sigma

Atto 655-Biotin

BioReagent, suitable for fluorescence, ≥95.0% (HPCE)

Synonym: Biotin-Atto 655

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Properties

Related Categories Biochemicals and Reagents, Biotin Conjugates, Fluorescent Conjugates, Fluorescent Probes, Labels, Particles and Stains
product line   BioReagent
assay   ≥95.0% (HPCE)
solubility   DMF: soluble
  DMSO: soluble
  acetonitrile: soluble
fluorescence   λex 655 nm; λem 680 nm in 0.1 M phosphate pH 7.0
suitability   suitable for fluorescence
storage temp.   −20°C

Description

General description

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Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Application

Atto 655 conjugated to biotin is useful for binding to proteins (i.e., avidin and streptavidin). The fluorescent properties include excellent thermal and photo-stability, high quantum yield, strong absorption (663 nm) and fluorescence (684 nm), and low triplet formation. It is a zwitterionic dye, carrying a net electrical charge of zero. Electron donors such as guanine and tryptophan can easily quench the fluorescence of this fluorophore. Biotin conjugated with ATTO 655 was applied to assess efficiency of BSA/avidin structures bound to a surface.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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