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08450 Sigma

7-Amino-4-methyl-3-coumarinacetic acid N-succinimidyl ester

BioReagent, suitable for fluorescence, ≥90% (HPLC)

Synonym: 7-(Dimethylamino)coumarin-4-acetic acid-NHS, 7-Amino-4-methyl-3coumarinylacetyl, N-Succinimidyl 7-amino-4-methyl-3-coumarinylacetate, AMCA-NHS, Succinimidyl-7-amino-4-methylcoumarin-3-acetate

  • CAS Number 113721-87-2

  • Empirical Formula (Hill Notation) C16H14N2O6

  • Molecular Weight 330.29

  •  Beilstein Registry Number 9007679

  •  MDL number MFCD00036170

  •  PubChem Substance ID 329748586

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Properties

Related Categories Biochemicals and Reagents, Fluorescent Indicators and Probes, Fluorescent Labels, Fluorescent Probes, Labels, Particles and Stains, Functional Group Reactive Labels,
InChI Key   ILKQVTIMOGNSAS-UHFFFAOYSA-N
product line   BioReagent
assay   ≥90% (HPLC)
solubility   DMF: soluble
  DMSO: soluble
fluorescence   λex 354 nm; λem 440 nm in methanol
suitability   suitable for fluorescence
storage temp.   −20°C

Description

Other Notes

Labeling of lysyl residues in proteins

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Application

AMCA-NHS has been utilized in the development of a donor europium chelate fluorochrome for use in LRET based analyses by first reacting with an amino group and then with DTPA anhydride . Utilized in FRET based studies, acting as the donor dye and can be paired with fluorescein-5-isothiocyanate as the acceptor dye . A blue fluorescent dye reactive towards primary amine groups on proteins, peptides, antibodies, and other biomolecules (at pH 7.0-9.0).

Price and Availability

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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