• USA Home
  • 08951 - 6-Carboxy-fluorescein diacetate N-succinimidyl ester

EMAIL THIS PAGE TO A FRIEND

08951 Sigma

6-Carboxy-fluorescein diacetate N-succinimidyl ester

for fluorescence, ≥90.0% (HPLC)

Synonym: 6-Carboxy-di-O-acetylfluorescein N-succinimidyl ester

Purchase

Properties

Related Categories Biochemicals and Reagents, Fluorescein and Derivatives, Fluorescence, Fluorescent Labeling of Amines, Fluorescent Labels,
grade   for fluorescence
assay   ≥90.0% (HPLC)
solubility   DMF: soluble
  DMSO: soluble
fluorescence   λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (after cleavage by esterase)
storage temp.   −20°C

Description

Other Notes

Reagent for continuously determining the intracellular pH in bacteria by fluorometry6; labeling human intervertebral disk cells in vitro for fluorescence microscopy7

Application

The succinimidyl ester group is capable of spontaneously and irreversibly binding to free amines1. Utilized in flow cytometry experiments for tracking cell division in both mammalian cells and bacteria2,3,4. Used in combination with several cell permeabilizers as an effective, nondestructive fluorescencent stain to quickly detect bacterial cells5.

Price and Availability

TruPAGE™ Pre-cast Polyacrylamide Gels


TruPAGE™ Pre-cast Polyacrylamide GelsTruPAGE Gels run true every time!

  • . 2-Year Shelf Life
  • . Superior Band Resolution
  • . Strong & Tear Resistant
  • . Higher Capacity Wells



Duolink Webinar

Safety & Documentation

Safety Information

WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

Peer-Reviewed Papers

References

Set your institution to view full text papers.

1. Use of CFSE staining of borreliae in studies on the interaction between borreliae and human neutrophils. Tuominen-Gustafsson, H., Penttinen, et al. BMC Microbiol. 6, 92, (2006)

Loading...


2. Flow cytometric analysis of Lactobacillus plantarum to monitor lag times, cell division and injury Ueckert, J.E., Nebe-von-Caron, G., et al. Lett. Appl. Microbiol. 25, 295, (1997)

Loading...


3. Tracking cell division using CFSE Hodgkin, P. D., and Lyons, A. B. Today's Life Sci. 12, 36, (2000)

4. Flow cytometric analysis of cell division history using dilution of carboxyfluorescein diacetate succinimidyl ester, a stability integrated fluorescent probe Lyons, A. B., Hasbold, J., and Hodgkin, P. D. Methods Cell Biol. 63, 375, (2001)

Loading...


5. Development of a nondestructive fluorescence-based enzymatic staining of microcolonies for enumerating bacterial contamination in filterable products Baumstummler, A., Chollet, R., Meder, H., et al. J. Appl. Microbiol. 110, 69, (2010)

Loading...


6. A Novel Method for Continuous Determination of the Intracellular pH in Bacteria with the Internally Conjugated Fluorescent Probe 5 (and 6-)-Carboxyfluorescein Succinimidyl Ester. P. Breeuwer et al. Appl. Environ. Microbiol. 62, 178, (1996)

Loading...


7. Optimization of 5-(and-6)-carboxyfluorescein diacetate succinimidyl ester for labeling human intervertebral disc cells in vitro. H.E. Gruber et al. Biotech. Histochem. 75, 118, (2000)

Loading...


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?