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  • 08951 - 6-Carboxy-fluorescein diacetate N-succinimidyl ester

08951 Sigma

6-Carboxy-fluorescein diacetate N-succinimidyl ester

for fluorescence, ≥90.0% (HPLC)

Synonym: 6-Carboxy-di-O-acetylfluorescein N-succinimidyl ester



Related Categories Biochemicals and Reagents, Fluorescein and Derivatives, Fluorescence, Fluorescent Labeling of Amines, Fluorescent Labels,
grade   for fluorescence
assay   ≥90.0% (HPLC)
solubility   DMF: soluble
  DMSO: soluble
fluorescence   λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (after cleavage by esterase)
storage temp.   −20°C


Other Notes

Reagent for continuously determining the intracellular pH in bacteria by fluorometry6; labeling human intervertebral disk cells in vitro for fluorescence microscopy7


The succinimidyl ester group is capable of spontaneously and irreversibly binding to free amines1. Utilized in flow cytometry experiments for tracking cell division in both mammalian cells and bacteria2,3,4. Used in combination with several cell permeabilizers as an effective, nondestructive fluorescencent stain to quickly detect bacterial cells5.

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Safety & Documentation

Safety Information

WGK Germany 


Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers


Set your institution to view full text papers.

1. Use of CFSE staining of borreliae in studies on the interaction between borreliae and human neutrophils. Tuominen-Gustafsson, H., Penttinen, et al. BMC Microbiol. 6, 92, (2006)


2. Flow cytometric analysis of Lactobacillus plantarum to monitor lag times, cell division and injury Ueckert, J.E., Nebe-von-Caron, G., et al. Lett. Appl. Microbiol. 25, 295, (1997)


3. Tracking cell division using CFSE Hodgkin, P. D., and Lyons, A. B. Today's Life Sci. 12, 36, (2000)

4. Flow cytometric analysis of cell division history using dilution of carboxyfluorescein diacetate succinimidyl ester, a stability integrated fluorescent probe Lyons, A. B., Hasbold, J., and Hodgkin, P. D. Methods Cell Biol. 63, 375, (2001)


5. Development of a nondestructive fluorescence-based enzymatic staining of microcolonies for enumerating bacterial contamination in filterable products Baumstummler, A., Chollet, R., Meder, H., et al. J. Appl. Microbiol. 110, 69, (2010)


6. A Novel Method for Continuous Determination of the Intracellular pH in Bacteria with the Internally Conjugated Fluorescent Probe 5 (and 6-)-Carboxyfluorescein Succinimidyl Ester. P. Breeuwer et al. Appl. Environ. Microbiol. 62, 178, (1996)


7. Optimization of 5-(and-6)-carboxyfluorescein diacetate succinimidyl ester for labeling human intervertebral disc cells in vitro. H.E. Gruber et al. Biotech. Histochem. 75, 118, (2000)


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