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08951 Sigma

6-Carboxy-fluorescein diacetate N-succinimidyl ester

for fluorescence, ≥90.0% (HPLC)

Synonym: 6-Carboxy-di-O-acetylfluorescein N-succinimidyl ester

Properties

Related Categories Biochemicals and Reagents, Fluorescein and Derivatives, Fluorescence, Fluorescent Labeling of Amines, Fluorescent Labels,
grade   for fluorescence
assay   ≥90.0% (HPLC)
solubility   DMF: soluble
  DMSO: soluble
fluorescence   λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (after cleavage by esterase)
storage temp.   −20°C

Description

Other Notes

Reagent for continuously determining the intracellular pH in bacteria by fluorometry6; labeling human intervertebral disk cells in vitro for fluorescence microscopy7

Application

The succinimidyl ester group is capable of spontaneously and irreversibly binding to free amines1. Utilized in flow cytometry experiments for tracking cell division in both mammalian cells and bacteria2,3,4. Used in combination with several cell permeabilizers as an effective, nondestructive fluorescencent stain to quickly detect bacterial cells5.

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