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09063001 Sigma

U-251 MG cell line human

Properties

biological source   Brain from human
growth mode   Adherent
karyotype   2n = 46
morphology   Pleomorphic/astrocytoid
products   Contains GFAP positive cells
receptors   PDGFR alpha and EGFR. See Nistér M. et al., (1988) & (1991)
application(s)   cell culture: suitable
shipped in   dry ice
storage temp.   −196°C

Description

General description

For complete product information, please see ECACC

Cell Line Origin

Human glioblastoma astrocytoma

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 10,11
D16S539: 12
D5S818: 11,12
D7S820: 10,12
THO1: 9.3
TPOX: 8
vWA: 16,18

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Cell Line Description

Derived from a malignant glioblastoma tumour by explant technique. U-251 was formerly distributed as U-373 MG (Sigma Catalogue number 89081403) until short tandem repeat (STR)-PCR profiling confirmed identity with U-251. A new deposit of U-373 MG known as U-373 MG (Uppsala) is now available (Sigma Catalogue number 08061901).Background to the identity query for the cell line U-373 MG: The American Type Culture Collection (ATCC) reported that their stock of U-373 MG had been shown to have differing genetic properties to stock from the originator′s laboratory, and to share similarities with another glioblastoma cell line, U-251. In light of this, ECACC undertook an investigation into the authenticity of its own stock of the U-373 MG cell line. ECACC found similar results to the ATCC i.e. the stock held as U-373 MG was found to be identical by STR-PCR profiling to U-251. The U-373 MG cell line listed under catalogue number 89081403 has been re-named as ′U-251 (formerly known as U-373 MG)′ and has the new Sigma Catalogue number 09063001.

Price and Availability

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