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09278 Sigma

4-[4-(Dimethylamino)phenylazo]benzoic acid N-succinimidyl ester

≥98.0% (HPLC)

Synonym: 4-(Dimethylamino)azobenzene-4′-carboxylic acid N-succinimidyl ester, DABCYL-N-succinimidyl ester

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Properties

Related Categories Biochemicals and Reagents, Fluorescent Labels, Fluorescent Probes, Labels, Particles and Stains, Luminescent Compounds/Detection, Other Fluorescent Labels,
InChI Key   IBOVDNBDQHYNJI-QZQOTICOSA-N
assay   ≥98.0% (HPLC)
solubility   DMF: soluble
  DMSO: soluble
fluorescence   λex 428 nm; λem 453 nm
storage temp.   −20°C

Description

Analysis Note

Absorption spectra shows a maximum at 453 nm (in methanol). After reaction with butylamine: max. absorption at 428 nm (Lit.)

Other Notes

N-terminal modification of peptides in automated synthesis

Application

A useful reagent (similar to MANCYL-SE) for labeling proteins or peptides through their amino-groups by forming stable peptide bonds. The succinimidyl ester is reactive with terminal amines or lysines of peptides and other nucleophiles for fluorescent studies of proteins. Its fluorescent properties (has a characteristic broad and intense visible absorption but has no fluorescence) make it an ideal long wavelength quencher and it has been utilized as an acceptor chromophore in FRET studies.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Fluorescent Labeling of Peptides

Labeling peptides with fluorescent dyes or other labels provides powerful tools for the investigation of biological relevant interactions like receptor-ligand-binding,1-3 protein structures,4-6 and e...
ChemFiles Volume 5 Article 12
Keywords: Alzheimer Disease, Biochemistry, Electrophoresis, Enzyme activity, Immobilization, Ligands, Methods, Microscopy, Peptide synthesis, Separation, Solid phase peptide synthesis, Solvents, Spectroscopy, Tools

Peer-Reviewed Papers
15

References

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