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10080904 Sigma

utr-1

Mouse monoclonal against human CD-120b

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   Mouse x Human Hybridoma
ImmunoDon   Mouse (Mus musculus) spleen
Myeloma   PAI
Reactivity   Human CD120b (TNF receptor p75)
application(s)   cell culture | mammalian: suitable
isotype   IgG1
shipped in   dry ice
storage temp.   −196°C

Description

Application

Sandwich ELISA for soluble p75 TNF-R. No competition with TNF.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Culture Medium

Iscoves Modified Dulbeccos Medium (IMDM) + 2 mM glutamine + 25 U/ml Interleukin-6 (IL-6) + 5-10% Fetal Bovine Serum (FBS). Where the cells are grown under static conditions, 0.02% EDTA may be required to dislodge them from the plastic.

Subculture Routine

Seed cultures between 1-3 x 105 cells/ml; Saturation density 8-10 x 105 5% CO2; 37 °C

Price and Availability


Flow Cytometry Instruments

All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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