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10112302 Sigma

Shmac 4 cell line

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Properties

biological source   Prostate from human
growth mode   Adherent
karyotype   Not specified
morphology   Epithelial
products   See Lang SH, et.al (2006)
receptors   Not specified
application(s)   cell culture | mammalian: suitable
shipped in   dry ice
storage temp.   −196°C

Description

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Professor. Norman J Maitland Director YCR Cancer Research Unit Department of Biology (Area 13) University of York Heslington York YO10 5DD

Cell Line Origin

Prostate cancer moderately well differentiated, early stage

DNA Profile

STR-PCR Data:

Amelogenin: X,Y
CSF1PO: 12
D13S317: 11
D16S539: 11,14
D5S818: 12,13
D7S820: 7,10
THO1: 7,9
TPOX: 8
vWA: 17,19

Culture Medium

Stemline Keratinocyte Medium II (Sigma S0196) + Stemline Keratinocyte Growth Supplement (Sigma S9945) + 2 mM Glutamine + 2% Fetal Bovine Serum (FBS). Addition of 2% serum was found to increase cell viability.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at approximately 3 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C. It is important to inactivate the trypsin with an equal volume of trypsin inhibitor (the culture medium contains too low a serum content to inactivate the trypsin). To remove all traces of trypsin and trypsin inhibitor pellet the cells by centrifugation and resuspend the cells in fresh medium. Media change every second day.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Flash Point(F) 
188.6 °F
Flash Point(C) 
87 °C

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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