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10115 Sigma

Amyloglucosidase from Aspergillus niger

lyophilized, powder, ~70 U/mg

Synonym: 1,4-α-D-Glucan glucohydrolase, Exo-1,4-α-glucosidase, Glucoamylase

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Properties

Related Categories 3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Carbohydrate Hydrolysis,
form   powder
quality   lyophilized
mol wt   Mr ~97,000
optimum temperature   50 °C
storage temp.   2-8°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

For glycogen determination in whole yeast cells; Synthesis of hetero-oligosaccharides by glucoamylase in reverse.

Unit Definition

One unit corresponds to the amount of enzyme which liberates 1 μmole of glucose per minute at pH 4.8 and 60 °C (starch acc. to Zulkowsky, Cat. No. 85642, as substrate).

Price and Availability


Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
What is the molecular weight of Product 10115,  Amyloglucosidase from Aspergillus niger?
Amyloglucosidase from A. niger exists in two isoforms with MWs of 69,810 and 89,130, as determined by SDS-PAGE. See: Starch, 34, 346 (1982).
Does Product 10115,  Amyloglucosidase from Aspergillus niger, contain any stabilizers or additives?
Fluka does not add any extending additives such as glucose or sucrose. Only sodium acetate buffer salts are added. It is unknown to Fluka if the starting material contained any additives.
How can a solution of Product 10115,  Amyloglucosidase from Aspergillus niger, be prepared?
A 1 mg/mL solution may be prepared in water, or in 10 mM sodium acetate buffer at approximately pH 4.8.
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Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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