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10771 Sigma

Histopaque®-1077

sterile-filtered, density: 1.077 g/mL

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Properties

Related Categories ACCUSPIN and Histopaque, Cell Biology, Density Gradient Media, Hematology and Histology, Histopaque More...
Quality Level   PREMIUM
sterility   sterile-filtered
shelf life   Expiry date on the label.
impurities   endotoxin, tested
density   1.077 g/mL
storage temp.   2-8°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Histopaque-1077 has been used for-
• the isolation of peripheral blood mononuclear cells (PBMCs) and bone marrow mononuclear cells (BMMCs)
• purification of mononuclear cells through centrifugation
• isolation of human lymphocytes
• isolation of breast cancer circulating tumor cells (CTCs) from whole blood

General description

Histopaque-1077 is a sterile, endotoxin tested solution of polysucrose and sodium diatrizoate, adjusted to a density of 1.077g/mL. This ready-to-use medium facilitates rapid recovery of viable lymphocytes and other mononuclear cells from small volumes of whole blood.

Legal Information

Histopaque is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability


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Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
nwg
Protocols & Articles

Articles

Histopaque® Troubleshooting Guide

The following recommendations for troubleshooting the use of Histopaque and ACCUSPIN™ in cell separation techniques have been compiled by Sigma-Aldrich® Technical Services. These are based on observa...
Mark Frei
BioFiles v6 n5, 14–16
Keywords: Adhesion, Anticoagulants, Cell culture, Centrifugation, Coagulation, Culture media, Sample preparations, Separation, Sterilizations

Protocols

FICOLL 400*

Ficoll 400 is a highly branched polymer formed by the copolymerization of sucrose and epichlorohydrin. Ficoll 400 is completely non-ionic. Because of the abundance of hydroxyl groups, Ficoll 400 is v...
Keywords: Cell culture, Centrifugation, Culture media, Dialysis, Electrophoresis, Fractionation, Growth factors, Nucleic acid hybridization, Separation, Southern blot, Substitutions

Peer-Reviewed Papers
15

References

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