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10831 Sigma

Histopaque®-1083

sterile-filtered, density: 1.083 g/mL

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Properties

Related Categories ACCUSPIN and Histopaque, Cell Biology, Centrifugation Media, Core Bioreagents, Density Gradient Media,
Quality Level   PREMIUM
sterility   sterile-filtered
shelf life   Expiry date on the label.
impurities   endotoxin, tested
density   1.083 g/mL
storage temp.   2-8°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

General description

A solution containing polysucrose and sodium diatrizoate, adjusted to a density of 1.083 g/mL. Facilitates the recovery of viable mononuclear cells from rats, mice, and other small mammals.

Legal Information

Histopaque is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability


Tissue Diagnostics

All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
nwg

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
Are there limitations when attempting to use Product 10831, Histopaque®-1083 with the various Accuspin products?
Yes.  There are some limitations.  Small amounts of blood cannot be processed in the Accuspin tubes.  At least 3 mls of whole peripheral blood is expected.  Without sufficient red cell numbers, the mononuclear band fails to adequately clear the barrier or frit found within the Accuspin tubes.
I find mononuclear cells separate well from human blood over Product 10831, Histopaque®-1083, but the cell recovery from rats and mice is lower.  Why?
The most common cause for separation problems with Histopaque® 1083 is the blood source.  Blood from rats and mice is harder to obtain than blood from a human.  Most users experiencing problems are collecting blood with a syringe.  It is relatively uncommon for users to call in about poor separation when using vacuum tubes.  Humanely sacrifice the animal if possible and immediately collect the blood from the aorta or vena cava. Collecting blood from a severed head is never acceptable.  Blood obtained from bleeding the tail is also generally unacceptable.  Tissue fluids and other extraneous debris will often result in an activation of the coagulation cascase.  Blood drawn into a vacuum tube is the best source of blood.  See the product insert for Histopaque® 1083 for the amount of anticoagulant to add to blood collected with a syringe.
Can Product 10831, Histopaque®-1083, be used with human blood?
Cells can be recovered from human blood using Histopaque® 1083.  However Sigma sells Histopaque® 1077 specifically for use with human blood.  Histopaque® 1077 is intended for In Vitro Diagnostic Use.  Histopaque® 1083 is intended for research use only with animal blood.
Is there any data for expected percent recovery with rat or mouse blood when using Product 10831, Histopaque®-1083?
No.  Histopaque® 1083 is a research use product and no studies have been performed to determine percent recovery.
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Protocols & Articles

Articles

Histopaque® Troubleshooting Guide

The following recommendations for troubleshooting the use of Histopaque and ACCUSPIN™ in cell separation techniques have been compiled by Sigma-Aldrich® Technical Services. These are based on observa...
Mark Frei
BioFiles v6 n5, 14–16
Keywords: Adhesion, Anticoagulants, Cell culture, Centrifugation, Coagulation, Culture media, Sample preparations, Separation, Sterilizations

Protocols

FICOLL 400*

Ficoll 400 is a highly branched polymer formed by the copolymerization of sucrose and epichlorohydrin. Ficoll 400 is completely non-ionic. Because of the abundance of hydroxyl groups, Ficoll 400 is v...
Keywords: Cell culture, Centrifugation, Culture media, Dialysis, Electrophoresis, Fractionation, Growth factors, Nucleic acid hybridization, Separation, Southern blot, Substitutions

Peer-Reviewed Papers
15

References

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