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11012002 Sigma

KYAE-1 cell line

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Properties

biological source   Oesophagus from human
growth mode   Adherent
karyotype   Not specified
morphology   Epithelial
products   P53 mutation c.455C>T
receptors   Not specified
application(s)   cell culture | mammalian: suitable
shipped in   dry ice
storage temp.   −196°C

Description

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Cell Line Origin

Distal oesophageal adenocarcinoma,

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11,12
D16S539: 11
D5S818: 10,13
D7S820: 11,12
THO1: 6
TPOX: 8
vWA: 14

Culture Medium

RPMI-1640:Hams F12 1:1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:5 i.e. seeding at 3 x 10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. There is a requirement to change the culture medium every 2-3 days.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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