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11371 Sigma

Avidin-Peroxidase Conjugate from hen egg white/horseradish

lyophilized, powder, almost white, avidin ~5 U/mg

Synonym: Avidin-POD

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Properties

form   powder
quality   lyophilized
impurities   ≥80 U/mg peroxidase (further activity)
color   almost white
storage temp.   −20°C

Description

Other Notes

Staining immunoblots with biotinylated primary antibodies

Preparation Note

Conjugate Description: ~1.5 moles peroxidase/mol avidin; MBS-linkage (Cat. No.No. 63173)

Unit Definition

1 U corresponds to the amount of protein which binds 1μg (+)-biotin at pH 7.5 further activity: peroxidase ≥80 U/mg [1U corresponds 1 U corresponds to the amount of enzyme which oxidizes 1 μmol ABTS (Cat. No. 11557) per minute at pH 6.0 and 25°C]

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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