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11557 Sigma

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt

≥98.0% (HPLC)

Synonym: AzBTS-(NH4)2, Diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate)

  • CAS Number 30931-67-0

  • Empirical Formula (Hill Notation) C18H24N6O6S4

  • Molecular Weight 548.68

  •  Beilstein Registry Number 7329461

  •  EC Number 250-396-6

  •  MDL number MFCD00010404

  •  PubChem Substance ID 329749485

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Properties

Related Categories Biochemicals and Reagents, Fluorescent Enzyme Substrates, Fluorescent Enzyme Substrates for Immunoassays, Fluorescent Probes, Labels, Particles and Stains
InChI Key   OHDRQQURAXLVGJ-AXMZSLBLSA-N
assay   ≥98.0% (HPLC)
impurities   ~5% water
solubility   H2O: soluble50 mg/mL, hazy, green
storage temp.   2-8°C

Description

Application

2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is green in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS). Recommended for ELISA (microwell) procedures, not recommended for membrane applications.

Other Notes

Chromogenic substrate for peroxidase; The ABTS-H2O2-peroxidase system in EIA

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Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
DL7002000
Protocols & Articles
Peer-Reviewed Papers
15

References

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