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14533 Sigma

bisBenzimide H 33342 trihydrochloride

for fluorescence, ≥97.0% (HPLC)

Synonym: 2′-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole trihydrochloride, HOE 33342, Hoechst 33342, bisBenzimide

  • CAS Number 23491-52-3

  • Empirical Formula (Hill Notation) C27H28N6O · 3HCl · xH2O

  • Molecular Weight 561.93 (anhydrous basis)

  •  Beilstein Registry Number 1234011

  •  EC Number 245-691-1

  •  MDL number MFCD00012678

  •  PubChem Substance ID 57647173

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Properties

Related Categories Alphabetical, Biochemicals and Reagents, Detection, Fluorescent Probes, Labels, Particles and Stains, Fluorescent Stains,
grade   for fluorescence
InChI Key   FYEVKHPLBHLWHK-UHFFFAOYSA-N
assay   ≥97.0% (HPLC)
fluorescence   λex 340 nm; λem 510 nm
  λex 355 nm; λem 465 nm in TE buffer; DNA
storage temp.   2-8°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry.
Excitation max. = 346 nm
Emission max. = 460 nm

bisBenzimide H 33342 trihydrochloride can be used to study biochemicals and reagents, fluorescent probes, labels, particles and stains, luminescent compounds and detection and wavelength index. bisBenzimide H 33342 trihydrochloride, Hoechst 33342, has been used in a study to investigate the effects of inorganic and organic arsenic compounds on human T-lymphoblastoid leukemia cells. Hoechst 33342 has also been used in a study to examine the effects of 9 calcium antagonists on ABCG2/BCRP-mediated resistance and transport in HeLa and SN-38-resistant HeLa (HeLa/SN100) cells, overexpressing ABCG2/BCRP.

Biochem/physiol Actions

Membrane-permeable, fluorescent DNA stains with low cytotoxicity that intercalate in A-T regions of DNA.

Other Notes

Suitable for vital DNA staining of a variety of cell types; As a probe of membrane permeability in mammalian cells

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.



Safety & Documentation

Safety Information

Symbol 
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
DT4200000

Documents

Certificate of Analysis

Protocols & Articles

Articles

Nancy-520 for DNA Detection and Quantitation

Nancy-520 is a fluorescent stain for double stranded DNA (dsDNA) on agarose electrophoresis gels, with higher sensitivity than ethidium bromide and an easy, fast, and robust staining procedure. It is...
Monika Baeumle
BioFiles 2009, 4.1, 4.
Keywords: Catalytic combustion detector, Electrophoresis

Peer-Reviewed Papers
15

References

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