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14871 Sigma

BICINE

BioUltra, ≥99.5% (T)

Synonym: N,N-Bis(2-hydroxyethyl)glycine

  • CAS Number 150-25-4

  • Empirical Formula (Hill Notation) C6H13NO4

  • Molecular Weight 163.17

  •  Beilstein Registry Number 1769362

  •  EC Number 205-755-1

  •  MDL number MFCD00004295

  •  PubChem Substance ID 57647225

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Properties

Related Categories Alphabetical, BioUltra Buffers, Biochemicals and Reagents, Biological Buffers, Zwitterionic More...
InChI Key   FSVCELGFZIQNCK-UHFFFAOYSA-N
product line   BioUltra
assay   ≥99.5% (T)
impurities   insoluble matter, passes filter test
ign. residue   ≤0.1%
loss   ≤0.1% loss on drying, 20 °C (HV)
pH   4.0-5.5 (25 °C, 1 M in H2O)
useful pH range   7.6 - 9.0
pKa (25 °C)   8.3
solubility   H2O: soluble1 M at 20 °C, clear, colorless
anion traces   chloride (Cl-): ≤50 mg/kg
  sulfate (SO42-): ≤50 mg/kg
cation traces   Al: ≤5 mg/kg
  As: ≤0.1 mg/kg
  Ba: ≤5 mg/kg
  Bi: ≤5 mg/kg
  Ca: ≤20 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤50 mg/kg
  Li: ≤5 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Mo: ≤5 mg/kg
  Na: ≤50 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Sr: ≤5 mg/kg
  Zn: ≤5 mg/kg
λ   1 M in H2O
UV absorption   λ: 260 nm Amax: 0.070
  λ: 280 nm Amax: 0.040

Description

Application

Recommended buffer for low temperature biochemical work. Used in the preparation of stable substrate solution for serum guanase determination. Spacer in plasma protein fractionation with Sephadex® by isotachophoresis.

BICINE can be used to study biological buffers and zwitterionic compounds. N,N-Bis(2-hydroxyethyl)glycine has been used in a study that characterized refined crystal structures of human Ca2+/Zn2+-binding S100A3 protein. N,N-Bis(2-hydroxyethyl)glycine has also been used in a study to inform F1 antigen assembly.

Legal Information

Sephadex is a registered trademark of GE Healthcare

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

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Peer-Reviewed Papers
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